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<p>A lab-in-a-fiber component was fabricated using an optical fiber and a fiber capillary. It was used in a test suspension of fluorescently labeled and unlabeled cells and enabled detection of the labeled cells. Subsequently the labeled cells were selectively collected via suction into the capillary. A novel sampling technique reduced photobleaching of the labeled cells, extending the measurement time. The collected cells remained viable for downstream analysis. This platform’s low fabrication cost, simplicity, compatibility with standard laboratory equipment, and capacity for fully automated cell capture highlights its potential for future applications in minimally invasive sample collection and point-of-care diagnostics. We demonstrate this LiF device to showcase the capability of optical fiber technology in creating low-cost, low-complexity cancer diagnostic devices. Furthermore, the LiF device holds promise for in vivo diagnostics, facilitating cell isolation and analysis.</p>

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Lab-in-a-Fiber detection and capture of cells

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月20日

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  • João C. Varela,
  • Achar V. Harish,
  • Pawel Maniewski,
  • Timothy Gibbon,
  • Oana Tudoran,
  • Rainer Heuchel,
  • Matthias Löhr,
  • Walter Margulis,
  • Aman Russom,
  • Fredrik Laurell

摘要

A lab-in-a-fiber component was fabricated using an optical fiber and a fiber capillary. It was used in a test suspension of fluorescently labeled and unlabeled cells and enabled detection of the labeled cells. Subsequently the labeled cells were selectively collected via suction into the capillary. A novel sampling technique reduced photobleaching of the labeled cells, extending the measurement time. The collected cells remained viable for downstream analysis. This platform’s low fabrication cost, simplicity, compatibility with standard laboratory equipment, and capacity for fully automated cell capture highlights its potential for future applications in minimally invasive sample collection and point-of-care diagnostics. We demonstrate this LiF device to showcase the capability of optical fiber technology in creating low-cost, low-complexity cancer diagnostic devices. Furthermore, the LiF device holds promise for in vivo diagnostics, facilitating cell isolation and analysis.