Scholarlink

<p>Kaempferol is an active compound found in traditional Chinese medicine epimedium soup, which exhibits potent anti-inflammatory and antioxidant properties. Nevertheless, the mechanism of action in rheumatoid arthritis remains unclear. This study constructed targets protein interaction networks by utilizing the String platform. The analysis of GO function and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment was performed on key target genes. Target gene validation was conducted through microarray analysis. Molecular docking was employed to evaluate the relationship between kaempferol and various key targets. In vitro experiments were conducted to elucidate kaempferol’s mechanism of action on rheumatoid arthritis. Topological analysis of the protein protein interaction (PPI) network identified 10 core targets. Mitogen activated protein kinase 8 (MAPK8), peroxisome proliferator-activated receptor gamma (PPARG), and nuclear factor kappa-B (NF-kB) were all differentially expressed in the microarray dataset and all belonged to the target genes of kaempferol. Furthermore, kaempferol exhibited the highest binding affinity for MAPK8. In vitro cellular experiments demonstrated that kaempferol suppressed autophagy, and ameliorated abnormal proliferation and inflammation in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) cells by activating the MAPK8/NOD-like receptor protein 3(NLRP3) signaling pathway.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Probing the molecular mechanism of kaempferol in relieving rheumatoid arthritis based on network pharmacology

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月12日

查看全文
  • Haomiao Liu,
  • Huangying Lu,
  • Xuefei Fan,
  • Suhuan Chen,
  • Xiaoyu Chen,
  • Weilu Gao

摘要

Kaempferol is an active compound found in traditional Chinese medicine epimedium soup, which exhibits potent anti-inflammatory and antioxidant properties. Nevertheless, the mechanism of action in rheumatoid arthritis remains unclear. This study constructed targets protein interaction networks by utilizing the String platform. The analysis of GO function and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment was performed on key target genes. Target gene validation was conducted through microarray analysis. Molecular docking was employed to evaluate the relationship between kaempferol and various key targets. In vitro experiments were conducted to elucidate kaempferol’s mechanism of action on rheumatoid arthritis. Topological analysis of the protein protein interaction (PPI) network identified 10 core targets. Mitogen activated protein kinase 8 (MAPK8), peroxisome proliferator-activated receptor gamma (PPARG), and nuclear factor kappa-B (NF-kB) were all differentially expressed in the microarray dataset and all belonged to the target genes of kaempferol. Furthermore, kaempferol exhibited the highest binding affinity for MAPK8. In vitro cellular experiments demonstrated that kaempferol suppressed autophagy, and ameliorated abnormal proliferation and inflammation in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) cells by activating the MAPK8/NOD-like receptor protein 3(NLRP3) signaling pathway.