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<p>On the basis of previous studies, the low-polar part of <i>Achyranthes aspera</i> L. (<i>A. aspera</i>) had strong anti-inflammatory activity. Three compounds were isolated from the low polarity fraction of <i>A. aspera</i>, and their structures were identified as α-spinasterol (<b>1</b>), 7,8-dihydrospinasterol (<b>2</b>), 22,23-dihydrospinasterol (<b>3</b>). Among them, the content of α-spinasterol (<b>1</b>) in <i>A. aspera</i> was higher in the spring and winter seasons through HPLC methods, ranging from 0.0085 to 0.0157%. Futhermore, in the LPS-induced RAW264.7 cells inflammation model, α-spinasterol significantly reduced the levels of cytokines such as IL-6, PGE2 and TNF-α, inhibited the expression of COX-2, 5-LOX, p-IKKβ, p-NFκB and p-IkBα proteins, and promoted the expression of Nrf2, HO-1 and NQO1 proteins. Therefore, this study showed that α-spinasterol can inhibit LPS-induced RAW264.7 cells inflammation, and its mechanism may be related to the inhibition of NF-κB pathway, activation of Nrf2 pathway, and reduction of excessive release of inflammatory factors.</p>

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α-spinasterol isolated from Achyranthes aspera L. ameliorates inflammation via NF-κB and Nrf2/HO-1 pathways

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月17日

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  • Qiongli Zeng,
  • Weiting Xiao,
  • Heng Zhang,
  • Wei Liu,
  • Xionglong Wang,
  • Zhen Li,
  • Yue Han,
  • Zhi Wang,
  • Shunxiang Li,
  • Jinwei Yang,
  • Wen Ouyang

摘要

On the basis of previous studies, the low-polar part of Achyranthes aspera L. (A. aspera) had strong anti-inflammatory activity. Three compounds were isolated from the low polarity fraction of A. aspera, and their structures were identified as α-spinasterol (1), 7,8-dihydrospinasterol (2), 22,23-dihydrospinasterol (3). Among them, the content of α-spinasterol (1) in A. aspera was higher in the spring and winter seasons through HPLC methods, ranging from 0.0085 to 0.0157%. Futhermore, in the LPS-induced RAW264.7 cells inflammation model, α-spinasterol significantly reduced the levels of cytokines such as IL-6, PGE2 and TNF-α, inhibited the expression of COX-2, 5-LOX, p-IKKβ, p-NFκB and p-IkBα proteins, and promoted the expression of Nrf2, HO-1 and NQO1 proteins. Therefore, this study showed that α-spinasterol can inhibit LPS-induced RAW264.7 cells inflammation, and its mechanism may be related to the inhibition of NF-κB pathway, activation of Nrf2 pathway, and reduction of excessive release of inflammatory factors.