Scholarlink

<p>The present work investigates virucidal and antiviral compounds in the extracts of seed embryos of a lotus, <i>Nelumbo nucifera</i>, and a Thai ginseng, <i>Kaempferia parviflora</i>. Separation of the extracts led to the identification of antiviral compounds against SARS-CoV-2. Neferine (<b>1</b>) and nuciferine (<b>3</b>) from <i>N. nucifera</i>, as well as their respective HCl salts (<b>2</b> and <b>4</b>), exhibited virucidal and antiviral activities against SARS-CoV-2. Virucidal activity of neferine salt (<b>2</b>) (EC<sub>50</sub> 4.78 µM) was 7.5 times better than its free-base, neferine (<b>1</b>) (EC<sub>50</sub> 36.01 µM), and the salt (<b>2</b>) also improved the selectivity index (SI), showing less cytotoxicity than <b>1</b>. This work demonstrates that organic salts have an impact on biological activities. A crude extract of <i>K. parviflora</i> rhizomes displayed virucidal activity (EC<sub>50</sub> 42.11&#xa0;µg/mL) and antiviral activity (EC<sub>50</sub> 39.28&#xa0;µg/mL). Isolation of a crude extract of <i>K. parviflora</i> rhizomes led to the identification of nine flavonoids (<b>5</b>–<b>13</b>). Among these flavonoids, only 5,7,4’-trimethoxyflavone (<b>8</b>) was found to show virucidal (EC<sub>50</sub> 437.90 µM) and antiviral (EC<sub>50</sub> 50.97 µM) activities against SARS-CoV-2. However, flavonoids (<b>5</b>–<b>13</b>) did not inhibit SARS-CoV-2 3CL<sup>pro</sup> enzyme at the concentrations of 10 µM and 100 µM. In conclusion, our data underscores the therapeutic potential of <i>N. nucifera</i> and <i>K. parviflora</i> derived bioactive compounds against SARS-CoV-2.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effects of organic salts of virucidal and antiviral compounds from Nelumbo nucifera and Kaempferia parviflora against SARS-CoV-2

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月21日

查看全文
  • Dan-Dan Yang,
  • Nopporn Chutiwitoonchai,
  • Feng Wang,
  • Ping Tian,
  • Sanya Sureram,
  • Xiaoguang Lei,
  • Chulabhorn Mahidol,
  • Somsak Ruchirawat,
  • Prasat Kittakoop

摘要

The present work investigates virucidal and antiviral compounds in the extracts of seed embryos of a lotus, Nelumbo nucifera, and a Thai ginseng, Kaempferia parviflora. Separation of the extracts led to the identification of antiviral compounds against SARS-CoV-2. Neferine (1) and nuciferine (3) from N. nucifera, as well as their respective HCl salts (2 and 4), exhibited virucidal and antiviral activities against SARS-CoV-2. Virucidal activity of neferine salt (2) (EC50 4.78 µM) was 7.5 times better than its free-base, neferine (1) (EC50 36.01 µM), and the salt (2) also improved the selectivity index (SI), showing less cytotoxicity than 1. This work demonstrates that organic salts have an impact on biological activities. A crude extract of K. parviflora rhizomes displayed virucidal activity (EC50 42.11 µg/mL) and antiviral activity (EC50 39.28 µg/mL). Isolation of a crude extract of K. parviflora rhizomes led to the identification of nine flavonoids (5–13). Among these flavonoids, only 5,7,4’-trimethoxyflavone (8) was found to show virucidal (EC50 437.90 µM) and antiviral (EC50 50.97 µM) activities against SARS-CoV-2. However, flavonoids (5–13) did not inhibit SARS-CoV-2 3CLpro enzyme at the concentrations of 10 µM and 100 µM. In conclusion, our data underscores the therapeutic potential of N. nucifera and K. parviflora derived bioactive compounds against SARS-CoV-2.