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<p>During transport through the oviduct, sperm interact with epithelial cells by attaching to specific glycans, a mechanism believed to select sperm and prolong their viability. An in vitro model of sperm-oviduct interactions was developed, consisting of a glass surface (either a slide or a coverslip) to which an oviduct glycan (sulfated Lewis X trisaccharide; suLe<sup>X</sup>) is coupled. The ability of porcine sperm to attach to suLe<sup>X</sup>-surfaces and detach in response to progesterone and mature cumulus-oocyte complexes (COCs) was validated. The suLe<sup>X</sup>-coverslip was adapted for in vitro fertilization (IVF), termed glycan-IVF, by allowing porcine sperm to first bind suLe<sup>X</sup> before transferring mature COCs. The glycan-IVF method produced a percentage of fertilized oocytes comparable to that of conventional IVF (75.1 vs. 72.0%). Finally, the ability of the suLe<sup>X</sup>-coverslip to maintain sperm fertilizing ability over time was assessed. After 24&#xa0;h of incubation, fertilization by sperm bound to the suLe<sup>X</sup>-coverslip was sustained, compared to sperm with unmodified coverslips (12.0 vs. 1.0%, <i>p</i> &lt; 0.05). Furthermore, the percentage of polyspermic zygotes was reduced in the suLe<sup>X</sup>-coverslip method (17.7 vs. 41.3%, <i>p</i> &lt; 0.05). This study validated an in vitro model for studying sperm-oviduct interactions, with potential applications in assisted reproductive technologies.</p>

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Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月8日

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  • Sandra Soto-Heras,
  • Larissa J. Volz,
  • Nicolai Bovin,
  • David J. Miller

摘要

During transport through the oviduct, sperm interact with epithelial cells by attaching to specific glycans, a mechanism believed to select sperm and prolong their viability. An in vitro model of sperm-oviduct interactions was developed, consisting of a glass surface (either a slide or a coverslip) to which an oviduct glycan (sulfated Lewis X trisaccharide; suLeX) is coupled. The ability of porcine sperm to attach to suLeX-surfaces and detach in response to progesterone and mature cumulus-oocyte complexes (COCs) was validated. The suLeX-coverslip was adapted for in vitro fertilization (IVF), termed glycan-IVF, by allowing porcine sperm to first bind suLeX before transferring mature COCs. The glycan-IVF method produced a percentage of fertilized oocytes comparable to that of conventional IVF (75.1 vs. 72.0%). Finally, the ability of the suLeX-coverslip to maintain sperm fertilizing ability over time was assessed. After 24 h of incubation, fertilization by sperm bound to the suLeX-coverslip was sustained, compared to sperm with unmodified coverslips (12.0 vs. 1.0%, p < 0.05). Furthermore, the percentage of polyspermic zygotes was reduced in the suLeX-coverslip method (17.7 vs. 41.3%, p < 0.05). This study validated an in vitro model for studying sperm-oviduct interactions, with potential applications in assisted reproductive technologies.