Scholarlink

<p>The DHHC domain genes are crucial for protein lipid modification, a key post-translational modification influencing membrane targeting, subcellular trafficking, and protein function. Despite their significance, the DHHC gene family in Saccharinae remains understudied. Here, we identified 32 (110 alleles), 28, 53, and 48 DHHC genes in <i>Saccharum spontaneum</i> Np-X, <i>Erianthus rufipilus</i>, <i>Miscanthus sinensis</i>, and <i>Miscanthus lutarioriparius</i>, respectively. Collinearity analysis uncovered the loss of two <i>M. lutarioriparius</i> genes, homologues of <i>EruDHHC1C</i> and <i>EruDHHC3A</i>. Phylogenetic and classification analyses categorized DHHC family members into six subgroups (A-F). Ka/Ks ratio analysis indicated that gene duplication in these species was primarily driven by whole-genome duplication (WGD) and dispersed duplication (DSD), with DHHC genes evolving under strong purifying selection. Gene expression and trait correlation analysis revealed a significant negative correlation between <i>SspDHHC28A</i> expression in <i>S. spontaneum</i> and sucrose content, suggesting a role in photosynthesis product transport during rapid growth. This study deepens our understanding of the DHHC gene family’s functional dynamics and evolutionary path in Saccharinae, laying a foundation for future research.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evolutionary analysis of the DHHCs in Saccharinae

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月17日

查看全文
  • Hao Wen,
  • Xinyu Liu,
  • Xueting Zhao,
  • Tingting Zhao,
  • Cuilian Feng,
  • Hailong Chang,
  • Jungang Wang,
  • Jishan Lin

摘要

The DHHC domain genes are crucial for protein lipid modification, a key post-translational modification influencing membrane targeting, subcellular trafficking, and protein function. Despite their significance, the DHHC gene family in Saccharinae remains understudied. Here, we identified 32 (110 alleles), 28, 53, and 48 DHHC genes in Saccharum spontaneum Np-X, Erianthus rufipilus, Miscanthus sinensis, and Miscanthus lutarioriparius, respectively. Collinearity analysis uncovered the loss of two M. lutarioriparius genes, homologues of EruDHHC1C and EruDHHC3A. Phylogenetic and classification analyses categorized DHHC family members into six subgroups (A-F). Ka/Ks ratio analysis indicated that gene duplication in these species was primarily driven by whole-genome duplication (WGD) and dispersed duplication (DSD), with DHHC genes evolving under strong purifying selection. Gene expression and trait correlation analysis revealed a significant negative correlation between SspDHHC28A expression in S. spontaneum and sucrose content, suggesting a role in photosynthesis product transport during rapid growth. This study deepens our understanding of the DHHC gene family’s functional dynamics and evolutionary path in Saccharinae, laying a foundation for future research.