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<p>Natriuretic peptides (NPs) have an important role in lipid metabolism in skeletal muscle and adipose tissue in animals. C-type natriuretic peptide (CNP) is an important NP, but the molecular mechanisms that underlie its activity are not completely understood. Treatment of intramuscular fat (IMF) and subcutaneous fat (SCF) adipocytes with CNP led to decreased differentiation, promoted proliferation and lipolysis, and increased the expression of natriuretic peptide receptor B (<i>NPRB</i>) mRNA. Silencing natriuretic peptide C (<i>NPPC</i>) had the opposite results in IMF and SCF adipocytes. Transcriptome analysis found 665 differentially expressed genes (DEGs) in IMF adipocytes and 991 in SCF adipocytes. Seven genes in IMF adipocytes (<i>FABP4</i>, <i>APOA1</i>, <i>ACOX2</i>, <i>ADIPOQ</i>, <i>CD36</i>, <i>FABP5</i>, and <i>LPL</i>) and eight genes in SCF adipocytes (<i>ACOX3</i>, <i>ACSL1</i>, <i>APOA1</i>, <i>CPT1A</i>, <i>CPT2</i>, <i>FABP4</i>, <i>PDPK1</i> and <i>PPARα</i>) are related to fat metabolism. Fifteen genes were found to be enriched in the peroxisome proliferator-activated receptor (PPAR) pathway. Integrated analysis identified 113 intersection genes in IMF and SCF adipocytes, two of which (<i>APOA1</i> and <i>FABP4</i>) were enriched in the PPAR pathway. In conclusion, CNP may regulated lipid metabolism through the <i>NPRB</i>-PPAR pathway in both IMF and SCF adipocytes, <i>FABP4</i> and <i>APOA1</i> may be the key genes that mediated CNP regulation of fat deposition.</p>

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C-type natriuretic peptide regulates lipid metabolism through a NPRB-PPAR pathway in the intramuscular and subcutaneous adipocytes in chickens

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月15日

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  • Huayun Huang,
  • Longzhou Liu,
  • Zhong Liang,
  • Qianbao Wang,
  • Chunmiao Li,
  • Zhengyang Huang,
  • Zhenhua Zhao,
  • Wei Han

摘要

Natriuretic peptides (NPs) have an important role in lipid metabolism in skeletal muscle and adipose tissue in animals. C-type natriuretic peptide (CNP) is an important NP, but the molecular mechanisms that underlie its activity are not completely understood. Treatment of intramuscular fat (IMF) and subcutaneous fat (SCF) adipocytes with CNP led to decreased differentiation, promoted proliferation and lipolysis, and increased the expression of natriuretic peptide receptor B (NPRB) mRNA. Silencing natriuretic peptide C (NPPC) had the opposite results in IMF and SCF adipocytes. Transcriptome analysis found 665 differentially expressed genes (DEGs) in IMF adipocytes and 991 in SCF adipocytes. Seven genes in IMF adipocytes (FABP4, APOA1, ACOX2, ADIPOQ, CD36, FABP5, and LPL) and eight genes in SCF adipocytes (ACOX3, ACSL1, APOA1, CPT1A, CPT2, FABP4, PDPK1 and PPARα) are related to fat metabolism. Fifteen genes were found to be enriched in the peroxisome proliferator-activated receptor (PPAR) pathway. Integrated analysis identified 113 intersection genes in IMF and SCF adipocytes, two of which (APOA1 and FABP4) were enriched in the PPAR pathway. In conclusion, CNP may regulated lipid metabolism through the NPRB-PPAR pathway in both IMF and SCF adipocytes, FABP4 and APOA1 may be the key genes that mediated CNP regulation of fat deposition.