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<p>The Asian Citrus Psyllid (ACP), <i>Diaphorina citri</i>, severely threatens citrus production worldwide by transmitting the greening (= Huanglongbing)—causing bacterium <i>Candidatus</i> Liberibacter asiaticus. There is growing evidence that the push-pull strategy is suitable to partially mitigate HLB by repelling ACP with transgenic plants engineered to produce repellents and attracting the vector to plants with a minimal disease transmission rate. Species that pull ACP away from commercial citrus plants have been identified, and transgenic plants that repel ACP have been developed. The concept of a repellent-producing plant was first demonstrated with an <i>Arabidopsis</i> line engineered to overexpress a gene controlling the synthesis of <i>β</i>-caryophyllene and other sesquiterpenes. We have analyzed the volatile organic compounds released by this <i>Arabidopsis</i> line and identified <i>α</i>-humulene, <i>α</i>-copaene, and trace amounts of <i>β</i>-elemene, in addition to <i>β</i>-caryophyllene. Behavioral measurements demonstrated that <i>α</i>-copaene repels ACP at doses ca. 100× lower than those needed for <i>β</i>-caryophyllene repellence. In contrast, <i>α</i>-humulene is innocuous at the level emitted by the transgenic plant. We confirmed that a mixture of the three sesquiterpenes in the ratio 1:100:10 repels ACP. Likewise, a commercial sample of copaiba oil containing the three sesquiterpenes, in a proportion similar to that in the transgenic plant, repelled ACP.</p>

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α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月28日

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  • Rodrigo Facchini Magnani,
  • Haroldo Xavier Linhares Volpe,
  • Rejane Angélica Grigio Luvizotto,
  • Tatiana Aparecida Mulinari,
  • Thiago Trevisoli Agostini,
  • Jairo Kenupp Bastos,
  • Victor Pena Ribeiro,
  • Michele Carmo-Sousa,
  • Nelson Arno Wulff,
  • Leandro Peña,
  • Walter S. Leal

摘要

The Asian Citrus Psyllid (ACP), Diaphorina citri, severely threatens citrus production worldwide by transmitting the greening (= Huanglongbing)—causing bacterium Candidatus Liberibacter asiaticus. There is growing evidence that the push-pull strategy is suitable to partially mitigate HLB by repelling ACP with transgenic plants engineered to produce repellents and attracting the vector to plants with a minimal disease transmission rate. Species that pull ACP away from commercial citrus plants have been identified, and transgenic plants that repel ACP have been developed. The concept of a repellent-producing plant was first demonstrated with an Arabidopsis line engineered to overexpress a gene controlling the synthesis of β-caryophyllene and other sesquiterpenes. We have analyzed the volatile organic compounds released by this Arabidopsis line and identified α-humulene, α-copaene, and trace amounts of β-elemene, in addition to β-caryophyllene. Behavioral measurements demonstrated that α-copaene repels ACP at doses ca. 100× lower than those needed for β-caryophyllene repellence. In contrast, α-humulene is innocuous at the level emitted by the transgenic plant. We confirmed that a mixture of the three sesquiterpenes in the ratio 1:100:10 repels ACP. Likewise, a commercial sample of copaiba oil containing the three sesquiterpenes, in a proportion similar to that in the transgenic plant, repelled ACP.