Scholarlink

<p>The coconut rhinoceros beetle (CRB) is an important pest of palms in the Asia/Pacific regions. A natural pathogen of CRB, Oryctes rhinoceros nudivirus (OrNV) has successfully been introduced into virus-free, invasive CRB populations in many Pacific islands leading to pest suppression. While PCR is currently used for virus monitoring, a more portable, simplified in-field method of virus detection would be helpful. Here, we developed a Recombinase Polymerase Amplification (RPA)-based assay to detect the partial <i>gp54</i> gene of OrNV (OrNV-RPA) using fluorescence-based chemistry. The assay has a high degree of specificity with no cross-reactivity to the genomic DNA of various insects, soil, and bacterial species commonly found in the environment. Additionally, the assay has a high level of sensitivity with the ability to detect approximately 1610 copies of the <i>gp54</i> gene (15&#xa0;min reaction). OrNV-RPA assay results showed high consistency with the current standard PCR-based assay, whether using purified DNA (95.4%) or crude lysate (93.5%) from CRB midgut tissue, validating the ability of the OrNV-RPA assay to detect virus-free and virus-infected CRB specimens. The newly developed RPA-based assay provides an option for rapid in-field detection of OrNV to help improve CRB management in the Pacific Islands and other regions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Recombinase polymerase amplification-based rapid detection of Oryctes rhinoceros nudivirus (OrNV) infection in coconut rhinoceros beetles

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月30日

查看全文
  • Sandeep K. Gupta,
  • Nicola K. Richards,
  • Tyler Regtien,
  • Mitchell K. Weston,
  • Paul H. Maclean,
  • Jeanne M. E. Jacobs,
  • Alasdair D. L. Noble,
  • Trevor A. Jackson,
  • Sean D. G. Marshall

摘要

The coconut rhinoceros beetle (CRB) is an important pest of palms in the Asia/Pacific regions. A natural pathogen of CRB, Oryctes rhinoceros nudivirus (OrNV) has successfully been introduced into virus-free, invasive CRB populations in many Pacific islands leading to pest suppression. While PCR is currently used for virus monitoring, a more portable, simplified in-field method of virus detection would be helpful. Here, we developed a Recombinase Polymerase Amplification (RPA)-based assay to detect the partial gp54 gene of OrNV (OrNV-RPA) using fluorescence-based chemistry. The assay has a high degree of specificity with no cross-reactivity to the genomic DNA of various insects, soil, and bacterial species commonly found in the environment. Additionally, the assay has a high level of sensitivity with the ability to detect approximately 1610 copies of the gp54 gene (15 min reaction). OrNV-RPA assay results showed high consistency with the current standard PCR-based assay, whether using purified DNA (95.4%) or crude lysate (93.5%) from CRB midgut tissue, validating the ability of the OrNV-RPA assay to detect virus-free and virus-infected CRB specimens. The newly developed RPA-based assay provides an option for rapid in-field detection of OrNV to help improve CRB management in the Pacific Islands and other regions.