Scholarlink

<p>The long-term occupational exposure of coal miners has caused serious harm to their health. The respiratory microbiota is increasingly recognized as an important driver of respiratory health, and changes in microbiota composition and function are recognized as key causes of metabolic disorders. However, the temporal characteristics of pharyngeal flora and plasma lipidomics of coal miners after dust exposure remain unclear. In this study, 22 coal mine workers who underwent occupational health examination in a hospital in Tangshan City, Hebei Province, China were selected as the research objects. Coal mine workers with no history of coal dust exposure were selected as the control group (C), and front-line miners with coal dust exposure records were divided into 3 groups according to their exposure years. They were short-term exposure to dust group (exposure to dust less than 10 years, D-1), medium-term exposure to dust group (exposure to dust 10 to 20 years, D-2), and long-term exposure to dust group (exposure to dust greater than 20 years, D-3). In this study, 16sRNA gene sequencing technology and lipid metabolomics were used to investigate the characteristics of pharyngeal microbial community and the changes of plasma lipid spectrum of coal miners with different years of exposure to dust. The results showed that with the extension of dust exposure time, the OUT number of pharyngeal microflora decreased gradually. In addition, short-term exposure to dust had no significant effect on the richness and diversity of microbial flora, and the richness was decreased in D-2 and D-3 groups, while the diversity was decreased in D-3 groups. The analysis of Phylum composition showed that Bacteroidota, Proteobacteria and Firmicutes were the common dominant bacteria of pharyngeal microflora in all pharyngeal swab samples, and the relative abundance of Bacteroidota in D-3 group was significantly increased (<i>P</i> &lt; 0.05). Genus composition analysis showed that the dominant species of pharyngeal microbiota included 10 species, including Prevotella, Neisseria, Lactobacillus, Porphyromonas, etc. The relative abundance of Prevotella in D-3 group was significantly increased (<i>P</i> &lt; 0.05). Tax4Fun analysis found that lipid Metabolism is changed in dust exposure group. In addition, lipid metabolomics results showed that the lipid profile of coal miners was changed, mainly manifested as Cer(d18:0/14:0) in dust exposed workers, and glycerophosphocholines were significantly enriched. These results suggest that long-term dust exposure leads to the imbalance of pharyngeal microflora and the change of differential lipid metabolites in coal miners.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The temporal characteristics of pharyngeal microbiota and plasma lipidomics by silica exposure in coal miner

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月29日

查看全文
  • Xiangyi Li,
  • Mengyi Yang,
  • Yue Zhang,
  • Fangze Wan,
  • Ziwei Zheng,
  • Hongli Wang,
  • Shengqiang Zou,
  • Heliang Liu,
  • Hailan He

摘要

The long-term occupational exposure of coal miners has caused serious harm to their health. The respiratory microbiota is increasingly recognized as an important driver of respiratory health, and changes in microbiota composition and function are recognized as key causes of metabolic disorders. However, the temporal characteristics of pharyngeal flora and plasma lipidomics of coal miners after dust exposure remain unclear. In this study, 22 coal mine workers who underwent occupational health examination in a hospital in Tangshan City, Hebei Province, China were selected as the research objects. Coal mine workers with no history of coal dust exposure were selected as the control group (C), and front-line miners with coal dust exposure records were divided into 3 groups according to their exposure years. They were short-term exposure to dust group (exposure to dust less than 10 years, D-1), medium-term exposure to dust group (exposure to dust 10 to 20 years, D-2), and long-term exposure to dust group (exposure to dust greater than 20 years, D-3). In this study, 16sRNA gene sequencing technology and lipid metabolomics were used to investigate the characteristics of pharyngeal microbial community and the changes of plasma lipid spectrum of coal miners with different years of exposure to dust. The results showed that with the extension of dust exposure time, the OUT number of pharyngeal microflora decreased gradually. In addition, short-term exposure to dust had no significant effect on the richness and diversity of microbial flora, and the richness was decreased in D-2 and D-3 groups, while the diversity was decreased in D-3 groups. The analysis of Phylum composition showed that Bacteroidota, Proteobacteria and Firmicutes were the common dominant bacteria of pharyngeal microflora in all pharyngeal swab samples, and the relative abundance of Bacteroidota in D-3 group was significantly increased (P < 0.05). Genus composition analysis showed that the dominant species of pharyngeal microbiota included 10 species, including Prevotella, Neisseria, Lactobacillus, Porphyromonas, etc. The relative abundance of Prevotella in D-3 group was significantly increased (P < 0.05). Tax4Fun analysis found that lipid Metabolism is changed in dust exposure group. In addition, lipid metabolomics results showed that the lipid profile of coal miners was changed, mainly manifested as Cer(d18:0/14:0) in dust exposed workers, and glycerophosphocholines were significantly enriched. These results suggest that long-term dust exposure leads to the imbalance of pharyngeal microflora and the change of differential lipid metabolites in coal miners.