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<p><i>Salmonella</i> Infantis is an emerging pathogen highly associated with antibiotic-resistant issues and has contributed to increasing foodborne illness in recent years. Thus, finding novel antimicrobial agents is urgent for the solution. The objective was to characterize a newly isolated <i>Epseptimavirus</i> phage with antimicrobial activity against multidrug-resistant <i>S.</i> Infantis strains. <i>Salmonella</i> phage vB_SalS-SIY1lw (or SIY1lw) is a new member of the phages belonging to the <i>Epseptimavirus</i> genus. SIY1lw contained the receptor binding protein (ORF 23) and tail fiber protein (ORF 43) genes—both associated with bacterial host recognition and binding—similar to that in <i>Salmonella</i> phage OSY-STA (the <i>Epseptimavirus</i> genus) and <i>Escherichia</i> phage DaisyDussoix (the <i>Tequintavirus</i> genus), respectively. For biological traits, SIY1lw has a latent period of 30&#xa0;min and an estimated burst size of 42 PFU/CFU. The phage was polyvalent against <i>S.</i> <i>enterica</i> (Infantis and Newport) and non-pathogenic <i>E. coli</i> strains. The in vitro antimicrobial activity test showed that the phage with MOI of 1,000 is the most effective in reducing <i>S.</i> Infantis FSIS7823 and FSIS4921 by 1 and 0.8 log, respectively, over the 6-h treatment at 25&#xa0;°C. These findings indicate that the new <i>Epseptimavirus</i> phage SIY1lw has future potential to develop an antimicrobial alternative to multidrug-resistant <i>S.</i> Infantis strains.</p>

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A new Epseptimavirus bacteriophage vB_SalS-SIY1lw as a potential antimicrobial alternative to multidrug-resistant Salmonella Infantis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月5日

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  • Yen-Te Liao,
  • Angela Voelker,
  • Abigail R. Arellano,
  • Yujie Zhang,
  • Leslie A. Harden,
  • Alexandra Salvador,
  • Vivian C. H. Wu

摘要

Salmonella Infantis is an emerging pathogen highly associated with antibiotic-resistant issues and has contributed to increasing foodborne illness in recent years. Thus, finding novel antimicrobial agents is urgent for the solution. The objective was to characterize a newly isolated Epseptimavirus phage with antimicrobial activity against multidrug-resistant S. Infantis strains. Salmonella phage vB_SalS-SIY1lw (or SIY1lw) is a new member of the phages belonging to the Epseptimavirus genus. SIY1lw contained the receptor binding protein (ORF 23) and tail fiber protein (ORF 43) genes—both associated with bacterial host recognition and binding—similar to that in Salmonella phage OSY-STA (the Epseptimavirus genus) and Escherichia phage DaisyDussoix (the Tequintavirus genus), respectively. For biological traits, SIY1lw has a latent period of 30 min and an estimated burst size of 42 PFU/CFU. The phage was polyvalent against S. enterica (Infantis and Newport) and non-pathogenic E. coli strains. The in vitro antimicrobial activity test showed that the phage with MOI of 1,000 is the most effective in reducing S. Infantis FSIS7823 and FSIS4921 by 1 and 0.8 log, respectively, over the 6-h treatment at 25 °C. These findings indicate that the new Epseptimavirus phage SIY1lw has future potential to develop an antimicrobial alternative to multidrug-resistant S. Infantis strains.