Scholarlink

<p>Soft X-ray tomography (SXT) enables native-contrast three-dimensional (3D) imaging of fully hydrated, cryogenically preserved biological samples, revealing ultrastructural details without the need for staining, embedding, or sectioning. Traditionally available only at synchrotron facilities, recent advances in laser-driven plasma sources have led to the development of compact soft X-ray microscopes. Achieving a resolution of 54&#xa0;nm full-pitch and tomogram acquisition times of 30&#xa0;min to two hours, we validate the system across a range of biologically relevant contexts, including protists, yeast, and mammalian cells containing polymeric and inorganic nanoparticles. These use cases establish the robustness of the laboratory based system for studying cell architecture, organelle interactions, and nanoparticle trafficking. By showing that a compact SXT system can achieve reliable high-resolution imaging across various cell types, this study highlights a major step toward making correlative cryogenic X-ray imaging broadly accessible in laboratory settings. Future developments will aim at enhanced throughput, deeper integration with correlative imaging modalities, and extension to more complex specimen types, including tissue.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Demonstrating soft X-ray tomography in the lab for correlative cryogenic biological imaging using X-rays and light microscopy

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月27日

查看全文
  • Stephen O’Connor,
  • David Rogers,
  • Maryna Kobylynska,
  • James A. Geraets,
  • Katja Thaysen,
  • Jacob Marcus Egebjerg,
  • Madeleen C. Brink,
  • Louisa Herbsleb,
  • Michaela Salakova,
  • Leon Fuchs,
  • Frauke Alves,
  • Claus Feldmann,
  • Axel Ekman,
  • Paul Sheridan,
  • William Fyans,
  • Tony McEnroe,
  • Fergal O’Reilly,
  • Kenneth Fahy,
  • Roland A. Fleck,
  • Daniel Wüstner,
  • Jeremy C. Simpson,
  • Andreas Walter,
  • Sergey Kapishnikov

摘要

Soft X-ray tomography (SXT) enables native-contrast three-dimensional (3D) imaging of fully hydrated, cryogenically preserved biological samples, revealing ultrastructural details without the need for staining, embedding, or sectioning. Traditionally available only at synchrotron facilities, recent advances in laser-driven plasma sources have led to the development of compact soft X-ray microscopes. Achieving a resolution of 54 nm full-pitch and tomogram acquisition times of 30 min to two hours, we validate the system across a range of biologically relevant contexts, including protists, yeast, and mammalian cells containing polymeric and inorganic nanoparticles. These use cases establish the robustness of the laboratory based system for studying cell architecture, organelle interactions, and nanoparticle trafficking. By showing that a compact SXT system can achieve reliable high-resolution imaging across various cell types, this study highlights a major step toward making correlative cryogenic X-ray imaging broadly accessible in laboratory settings. Future developments will aim at enhanced throughput, deeper integration with correlative imaging modalities, and extension to more complex specimen types, including tissue.