<p>Liver fibrosis is a characteristic pathological feature of various chronic liver diseases, which is almost irreversible and intractable. Although many natural components have been shown to have therapeutic effects on liver fibrosis, no studies have examined the effects of Vitisin A on liver fibrosis and the molecular mechanisms involved. In our study, we demonstrated that Vitisin A inhibits liver fibrosis in a concentration- dependent and time-dependent manner. We found that Vitisin A inhibits the Nrf2/HO-1 pathway while inhibiting cuproptosis. We activated cuproptosis and inhibited Nrf2 expression separately, and found the inhibition of hepatic fibrosis by Vitisin A was blocked. The inhibitory effect of Vitisin A on mice model of liver fibrosis was also observed. Interestingly, Vitisin A significantly restored cell viability in liver injury cell model. In conclusion, this study suggests that Vitisin A is a promising therapeutic drug for the treatment of liver fibrosis.</p>