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<p>Peritoneal dialysis-related peritonitis (PDRP) is a major complication of home-based dialysis, often leading to catheter failure, hospitalization, or mortality. Current diagnostic approaches such as culture and Gram staining are slow, labor-intensive, and prone to false negatives. Here we report a culture-free triplex fluorescence aptasensor for the simultaneous detection of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>, two key pathogens in PDRP. The sensor design is based on a triplex DNA structure comprising an unlabelled aptamer flanked by fluorophore- and quencher-labeled complementary strands. In the presence of target bacteria, aptamer binding disrupts the triplex, leading to fluorescence restoration. The aptasensors demonstrated excellent sensitivity, high specificity against non-target organisms, and robust performance in sterilized clinical peritoneal fluid. This simple, cost-effective assay shows strong potential for rapid point-of-care diagnostics in peritoneal dialysis, enabling rapid identification of pathogens and timely clinical decision-making.</p>

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Design of a triplex fluorescence aptasensor for a culture-free diagnosis of peritoneal dialysis-related peritonitis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月17日

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  • Awatef Altabban,
  • Amina Rhouati,
  • Areej I. Alhazmi,
  • Dana Cialla-May,
  • Jürgen Popp,
  • Mohammed Zourob

摘要

Peritoneal dialysis-related peritonitis (PDRP) is a major complication of home-based dialysis, often leading to catheter failure, hospitalization, or mortality. Current diagnostic approaches such as culture and Gram staining are slow, labor-intensive, and prone to false negatives. Here we report a culture-free triplex fluorescence aptasensor for the simultaneous detection of Staphylococcus aureus and Pseudomonas aeruginosa, two key pathogens in PDRP. The sensor design is based on a triplex DNA structure comprising an unlabelled aptamer flanked by fluorophore- and quencher-labeled complementary strands. In the presence of target bacteria, aptamer binding disrupts the triplex, leading to fluorescence restoration. The aptasensors demonstrated excellent sensitivity, high specificity against non-target organisms, and robust performance in sterilized clinical peritoneal fluid. This simple, cost-effective assay shows strong potential for rapid point-of-care diagnostics in peritoneal dialysis, enabling rapid identification of pathogens and timely clinical decision-making.