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<p>The development of enzyme-free uric acid (UA) detection methods remains challenging due to cost, stability, and sensitivity limitations. Here, we report a uricase-free colorimetric biosensor using cobalt-1,3,5-benzene tricarboxylate metal–organic frameworks (CoBTC MOFs) with intrinsic peroxidase-like activity. CoBTC catalyzes the oxidation of TMB by conducting H₂O₂ to blue oxTMB, while UA reduces oxTMB to colorless TMB, allowing for the quantification of UA via absorbance changes. This biosensor achieved a detection limit of 0.25 μM and a linear range of 0.4–180 μM, outperforming many uricase-dependent assays. Clinical validation with human serum (recovery: 98–103%, RSD &lt; 3%) confirmed the resistance to matrix effects. This work provides a scalable and low-cost alternative for point-of-care UA monitoring in diagnostic settings.</p>

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Uricase-free and cost-effective colorimetric assay for uric acid based on CoBTC metal–organic frameworks

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月5日

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  • Shokooh Sadat Khaloo,
  • Mohammad Hossein Vaziri,
  • Fatemeh Azizi

摘要

The development of enzyme-free uric acid (UA) detection methods remains challenging due to cost, stability, and sensitivity limitations. Here, we report a uricase-free colorimetric biosensor using cobalt-1,3,5-benzene tricarboxylate metal–organic frameworks (CoBTC MOFs) with intrinsic peroxidase-like activity. CoBTC catalyzes the oxidation of TMB by conducting H₂O₂ to blue oxTMB, while UA reduces oxTMB to colorless TMB, allowing for the quantification of UA via absorbance changes. This biosensor achieved a detection limit of 0.25 μM and a linear range of 0.4–180 μM, outperforming many uricase-dependent assays. Clinical validation with human serum (recovery: 98–103%, RSD < 3%) confirmed the resistance to matrix effects. This work provides a scalable and low-cost alternative for point-of-care UA monitoring in diagnostic settings.