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<p>Potato virus Y (PVY) is one of the most significant pathogens in the production of cultivated potato <i>Solanum tuberosum</i> worldwide. The most efficient way to combat viral diseases is the use of resistant varieties. The <i>Ry</i><sub><i>chc</i></sub> gene was discovered in the tuber-bearing wild potato relative <i>Solanum chacoense</i>. This gene is able to confer extreme resistance to PVY by rapidly halting virus multiplication in plants without the development of visible symptoms. Two molecular markers were developed to predict the presence of the <i>Ry</i><sub><i>chc</i></sub> gene and PVY resistance with 100% accuracy. In this study, the diversity of the <i>Ry</i><sub><i>chc</i></sub> gene and its relationship with resistance to PVY were investigated in genotypes from the Russian potato genbank. A collection of 60 genotypes originating from different populations was examined for PVY resistance and for the presence of <i>Ry</i><sub><i>chc</i></sub> using molecular markers. The observed phenotypes ranged from susceptibility to hypersensitive reaction and extreme resistance to PVY. Irrespective of the type of resistance, all the PVY-resistant plants carried the <i>Ry</i><sub><i>chc</i></sub> gene, indicating the prevalence of this resistance mechanism in <i>S. chacoense</i>. Unexpectedly, two <i>S. chacoense</i> individuals with the <i>Ry</i><sub><i>chc</i></sub> gene were found to be completely susceptible to PVY. <i>Ry</i><sub><i>chc</i></sub> sequences were evaluated via long-read targeted sequencing in all <i>Ry</i><sub><i>chc</i></sub>-positive genotypes. Despite the low overall sequence variation observed in <i>Ry</i><sub><i>chc</i></sub> gene, identical polymorphisms were found in <i>Ry</i><sub><i>chc</i></sub> alleles from susceptible <i>S. chachoense</i> genotypes, demonstrating the existence of nonfunctional <i>Ry</i><sub><i>chc</i></sub> allele unable to confer PVY resistance.</p>

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Role of Rychc gene diversity in the resistance of wild potato relative Solanum chacoense to potato virus Y

  • 期刊论文

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  • 发表日期：

    2025年11月26日

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  • Aleksandr Antipov,
  • Alena Gurina,
  • Nikolay Zlobin,
  • Elena Rogozina

摘要

Potato virus Y (PVY) is one of the most significant pathogens in the production of cultivated potato Solanum tuberosum worldwide. The most efficient way to combat viral diseases is the use of resistant varieties. The Rychc gene was discovered in the tuber-bearing wild potato relative Solanum chacoense. This gene is able to confer extreme resistance to PVY by rapidly halting virus multiplication in plants without the development of visible symptoms. Two molecular markers were developed to predict the presence of the Rychc gene and PVY resistance with 100% accuracy. In this study, the diversity of the Rychc gene and its relationship with resistance to PVY were investigated in genotypes from the Russian potato genbank. A collection of 60 genotypes originating from different populations was examined for PVY resistance and for the presence of Rychc using molecular markers. The observed phenotypes ranged from susceptibility to hypersensitive reaction and extreme resistance to PVY. Irrespective of the type of resistance, all the PVY-resistant plants carried the Rychc gene, indicating the prevalence of this resistance mechanism in S. chacoense. Unexpectedly, two S. chacoense individuals with the Rychc gene were found to be completely susceptible to PVY. Rychc sequences were evaluated via long-read targeted sequencing in all Rychc-positive genotypes. Despite the low overall sequence variation observed in Rychc gene, identical polymorphisms were found in Rychc alleles from susceptible S. chachoense genotypes, demonstrating the existence of nonfunctional Rychc allele unable to confer PVY resistance.