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<p>Global foodborne <i>Salmonella</i> outbreaks pose a serious concern for food safety and public health underscoring the need for isolation of lytic phages with potential as biocontrol agents. A lytic bacteriophage vB_Sal_S6 was isolated which targets <i>Salmonella</i> serovars - Typhimurium, Bovismorbificans, Infantis, Typhi, Paratyphi, Weltevreden, Poona and as well as <i>Escherichia coli</i>. It exhibited strong bacteriolytic activity across a range of MOIs (0.01–10), with stability across pH 4–10 and temperature conditions (25–55&#xa0;°C). In artificially contaminated milk, vB_Sal_S6 reduced <i>Salmonella</i> counts by 3.9log at RT and 2.4log at 4&#xa0;°C within 9 and 6&#xa0;h respectively (MOI 10). Genomic characterization revealed a dsDNA genome of 36,127&#xa0;bp length and the absence of virulence, integrase, excisionase, and antibiotic resistance genes, supporting its suitability for biocontrol. Phylogenetic analysis indicated that vB_Sal_S6 clustered with other <i>Salmonella/E.coli</i> phages in <i>Tequintavirus</i> genus within <i>Demerecviridae</i> family. Docking studies identified that ORF123-encoded receptor-binding tail protein binds with ferrichrome receptor FhuA in <i>Salmonella</i> and <i>E.coli</i> as putative receptor. These findings establish vB_Sal_S6 as a promising candidate for biocontrol applications against <i>Salmonella</i> and <i>E. coli</i>.</p>

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Genomic characterization of novel lytic phage vB_Sal_S6 with putative host FhuA interaction and its application for Salmonella biocontrol in milk

  • 期刊论文

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  • 发表日期：

    2025年11月19日

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  • Ann Mary Isaac,
  • Pallavi K.P,
  • Chinmayi Nandkishor Mhatre,
  • Joseph Kingston. J.

摘要

Global foodborne Salmonella outbreaks pose a serious concern for food safety and public health underscoring the need for isolation of lytic phages with potential as biocontrol agents. A lytic bacteriophage vB_Sal_S6 was isolated which targets Salmonella serovars - Typhimurium, Bovismorbificans, Infantis, Typhi, Paratyphi, Weltevreden, Poona and as well as Escherichia coli. It exhibited strong bacteriolytic activity across a range of MOIs (0.01–10), with stability across pH 4–10 and temperature conditions (25–55 °C). In artificially contaminated milk, vB_Sal_S6 reduced Salmonella counts by 3.9log at RT and 2.4log at 4 °C within 9 and 6 h respectively (MOI 10). Genomic characterization revealed a dsDNA genome of 36,127 bp length and the absence of virulence, integrase, excisionase, and antibiotic resistance genes, supporting its suitability for biocontrol. Phylogenetic analysis indicated that vB_Sal_S6 clustered with other Salmonella/E.coli phages in Tequintavirus genus within Demerecviridae family. Docking studies identified that ORF123-encoded receptor-binding tail protein binds with ferrichrome receptor FhuA in Salmonella and E.coli as putative receptor. These findings establish vB_Sal_S6 as a promising candidate for biocontrol applications against Salmonella and E. coli.