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<p>Accurate separation of sexes is pivotal for the success of Sterile Insect Technique (SIT) and Incompatible Insect Technique (IIT) mosquito control programmes. We present a novel approach utilising a 15% sodium chloride (NaCl) treatment coupled with an automated mechanical separation system to significantly reduce female contamination among released males. Our findings not only demonstrated a 30-fold reduction in female contamination rates but also offer insights into the effects of salinity on mosquito larvae and pupae. This integrated strategy enhances the efficiency of large-scale mosquito production for disease control programmes.</p>

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Enhanced mosquito sex separation using NaCl for optimised sterile insect technique and incompatible insect technique programmes

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月28日

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  • Kee Kee Chng,
  • Jonathan Wee Kent Liew,
  • Sook Chin Ang,
  • Muhammad Faizal Zulkifli,
  • Chee-Seng Chong,
  • Cheong Huat Tan,
  • Deng Lu

摘要

Accurate separation of sexes is pivotal for the success of Sterile Insect Technique (SIT) and Incompatible Insect Technique (IIT) mosquito control programmes. We present a novel approach utilising a 15% sodium chloride (NaCl) treatment coupled with an automated mechanical separation system to significantly reduce female contamination among released males. Our findings not only demonstrated a 30-fold reduction in female contamination rates but also offer insights into the effects of salinity on mosquito larvae and pupae. This integrated strategy enhances the efficiency of large-scale mosquito production for disease control programmes.