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<p>Alterations in the abundance of <i>Bacteroides</i> species are linked to the disruption of the intestinal epithelial barrier and chronic inflammation and has been increasingly recognized as a factor in the development of neurological and neuropsychiatric disorders. Nevertheless, the exact role of <i>Bacteroides</i> species in the gut-brain cross-talk is still largely unexplored. Here, we investigated the immunoregulatory and neuromodulatory potential of two poorly characterized <i>Bacteroides</i> species, including <i>Bacteroides cellulosilyticus</i> and <i>Bacteroides xylanisolvens</i>. The results revealed that both <i>Bacteroides</i> isolates reduced inflammation in Caco-2 intestinal epithelial cells by decreasing the level of IL-8 chemokine and transcription of NF-kB, the two key factors involved in gut inflammation development and barrier disruption. In addition, the <i>Bacteroides</i> strains in the co-culture of Caco-2 cells and phytohemagglutinin-stimulated PBMCs reduced the production of pro-inflammatory cytokines TNF-α and IL-1β, as well as Th1-polarizing IFN-γ cytokine. Finally, in <i>Caenorhabditis elegans</i>,<i> Bacteroides</i> strains differently modulated the expression of the genes implicated in GABA, serotonin and dopamine signaling and synaptic vesicles release pointed to the strain-specific effects of these isolates on neural function. Altogether, these in vitro results show that tested <i>Bacteroides</i> strains may exert anti-inflammatory and neuromodulatory effects, indicating their potential role in microbiota-gut-brain axis.</p>

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Insight into immunoregulatory and neuromodulatory capability of Bacteroides cellulosilyticus and Bacteroides xylanisolvens human gut microbiota isolates

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月30日

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  • Miroslav Dinić,
  • Jelena Đokić,
  • Stefan Jakovljević,
  • Emilija Brdarić,
  • Hristina Mitrović,
  • Aleksandar Bisenić,
  • Dušan Radojević,
  • Svetlana Soković Bajić,
  • Sergej Tomić,
  • Nataša Golić

摘要

Alterations in the abundance of Bacteroides species are linked to the disruption of the intestinal epithelial barrier and chronic inflammation and has been increasingly recognized as a factor in the development of neurological and neuropsychiatric disorders. Nevertheless, the exact role of Bacteroides species in the gut-brain cross-talk is still largely unexplored. Here, we investigated the immunoregulatory and neuromodulatory potential of two poorly characterized Bacteroides species, including Bacteroides cellulosilyticus and Bacteroides xylanisolvens. The results revealed that both Bacteroides isolates reduced inflammation in Caco-2 intestinal epithelial cells by decreasing the level of IL-8 chemokine and transcription of NF-kB, the two key factors involved in gut inflammation development and barrier disruption. In addition, the Bacteroides strains in the co-culture of Caco-2 cells and phytohemagglutinin-stimulated PBMCs reduced the production of pro-inflammatory cytokines TNF-α and IL-1β, as well as Th1-polarizing IFN-γ cytokine. Finally, in Caenorhabditis elegans, Bacteroides strains differently modulated the expression of the genes implicated in GABA, serotonin and dopamine signaling and synaptic vesicles release pointed to the strain-specific effects of these isolates on neural function. Altogether, these in vitro results show that tested Bacteroides strains may exert anti-inflammatory and neuromodulatory effects, indicating their potential role in microbiota-gut-brain axis.