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<p>Dengue virus (DENV) infection in children exhibits varied clinical presentations, wherein the role of monocytes is important in the innate immune response. In this study, laboratory-confirmed DENV pediatric patients (<i>n</i> = 120), with DENV-2 infection, were categorized into dengue fever (DF), dengue with warning signs (DWS) and severe dengue (SD) were assessed for monocyte subpopulation analysis using immunophenotyping involving CD14 and CD16 host-surface markers. Molecular docking was performed using HADDOCK 2.4 to analyze the interactions between CD14, CD16 and DENV envelope and capsid proteins. Among the cases, 84 (70%) were classified as DF and 36 (30%) as DWS &amp; SD. Hematological and biochemical parameters indicated that thrombocytopenia and elevated hematocrit (&gt; 40%) were significantly more common in DWS &amp; SD, with markedly elevated liver enzymes (ALT and AST) in severe cases. Classical monocytes (CM-CD14++ CD16−) constituted 72.51% and 66.25% of the monocyte population in DF and DWS &amp; SD cases, respectively. Intermediate monocytes (IM-CD14+ CD16+) comprised 9.89% and 30.86% in DF and DWS &amp; SD cases, respectively. Non-classical monocytes (NCM-CD14+ CD16++) comprised 5.75% and 8.12% in DWS &amp; SD and DF cases, respectively. In silico analysis revealed host CD16 and CD14 exhibited potential interactions with DENV capsid and envelope proteins, with binding energies − 8.9, −&#xa0;10.1, −&#xa0;8.6, and − 11.1&#xa0;kcal/mol, respectively. IM was significantly increased in DWS &amp; SD compared to DF (<i>p</i> &lt; 0.05). These findings suggest that IM could act as host markers of DENV severity in children.</p>

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Association of intermediate monocytes with dengue severity among the pediatric population of Odisha, India

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月23日

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  • Manoj Kumar Dash,
  • Sagnika Samal,
  • Shailesh Rout,
  • Rajesh Kumar Bhola,
  • Janeka Gartia,
  • Ishani Saha,
  • Chinmay Kumar Behera,
  • Hamida Hamdi,
  • Mahesh Chandra Sahu,
  • Biswadeep Das

摘要

Dengue virus (DENV) infection in children exhibits varied clinical presentations, wherein the role of monocytes is important in the innate immune response. In this study, laboratory-confirmed DENV pediatric patients (n = 120), with DENV-2 infection, were categorized into dengue fever (DF), dengue with warning signs (DWS) and severe dengue (SD) were assessed for monocyte subpopulation analysis using immunophenotyping involving CD14 and CD16 host-surface markers. Molecular docking was performed using HADDOCK 2.4 to analyze the interactions between CD14, CD16 and DENV envelope and capsid proteins. Among the cases, 84 (70%) were classified as DF and 36 (30%) as DWS & SD. Hematological and biochemical parameters indicated that thrombocytopenia and elevated hematocrit (> 40%) were significantly more common in DWS & SD, with markedly elevated liver enzymes (ALT and AST) in severe cases. Classical monocytes (CM-CD14++ CD16−) constituted 72.51% and 66.25% of the monocyte population in DF and DWS & SD cases, respectively. Intermediate monocytes (IM-CD14+ CD16+) comprised 9.89% and 30.86% in DF and DWS & SD cases, respectively. Non-classical monocytes (NCM-CD14+ CD16++) comprised 5.75% and 8.12% in DWS & SD and DF cases, respectively. In silico analysis revealed host CD16 and CD14 exhibited potential interactions with DENV capsid and envelope proteins, with binding energies − 8.9, − 10.1, − 8.6, and − 11.1 kcal/mol, respectively. IM was significantly increased in DWS & SD compared to DF (p < 0.05). These findings suggest that IM could act as host markers of DENV severity in children.