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<p>The global transition towards sustainable energy necessitates rapid innovations in renewable technology, with biomethanation emerging as a promising approach. Methanogenic archaea, notably <i>Methanothermobacter marburgensis</i>, play a pivotal role in the biogenesis of biomethane as a renewable energy vector. This research introduces a new second-generation Simultaneous Bioreactor System (SBRS-II), a high-pressure cultivation platform engineered to optimize microbial gas fermentation. A novel sulfate-based growth medium (MM-CF-S) was formulated to substitute conventional chloride salts, thereby minimizing corrosion risks in steel reactors and eliminating the toxic compound NiCl<sub>2</sub>·6&#xa0;H<sub>2</sub>O, which enhances safety during large-scale deployment. Comparative analyses indicate that the new medium markedly boosts methane production rates, attaining a maximum of 285.86 ± 22.94&#xa0;mmol L<sup>−1</sup>&#xa0;h<sup>−1</sup>, approximately doubling the baseline. Additionally, metrics of biomass accumulation and specific methane productivity observed improvements, with <InlineEquation ID="IEq1"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="41598_2025_19544_Article_IEq1.gif" Format="GIF" Height="14" Rendition="HTML" Resolution="72" Type="Linedraw" Width="32" /> </InlineMediaObject> <EquationSource Format="TEX">\(q_{{{\text{CH}}_{{4}} }}\)</EquationSource> </InlineEquation> reaching 216.85 ± 17.54&#xa0;mmol g<sup>−1</sup>&#xa0;h<sup>−1</sup>. The sulfate medium also preserved pH stability under high-pressure conditions, ensuring physiological viability essential for <i>M. marburgensis</i> proliferation. Integration of the SBRS-II platform with the MM-CF-S medium signifies a significant advancement toward scalable, efficient biomethanation technologies, highlighting the potential of methanogenic archaea in renewable energy applications and establishing a foundation for further process optimization to meet industrial-scale demands.</p>

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A high-pressure bioreactor system for the cultivation of Methanothermobacter marburgensis on advanced growth media for sustainable energy applications

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月10日

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  • Marco Orthofer,
  • Walter Hofmann,
  • Simon K.-M. R. Rittmann,
  • Christian Paulik

摘要

The global transition towards sustainable energy necessitates rapid innovations in renewable technology, with biomethanation emerging as a promising approach. Methanogenic archaea, notably Methanothermobacter marburgensis, play a pivotal role in the biogenesis of biomethane as a renewable energy vector. This research introduces a new second-generation Simultaneous Bioreactor System (SBRS-II), a high-pressure cultivation platform engineered to optimize microbial gas fermentation. A novel sulfate-based growth medium (MM-CF-S) was formulated to substitute conventional chloride salts, thereby minimizing corrosion risks in steel reactors and eliminating the toxic compound NiCl2·6 H2O, which enhances safety during large-scale deployment. Comparative analyses indicate that the new medium markedly boosts methane production rates, attaining a maximum of 285.86 ± 22.94 mmol L−1 h−1, approximately doubling the baseline. Additionally, metrics of biomass accumulation and specific methane productivity observed improvements, with \(q_{{{\text{CH}}_{{4}} }}\) reaching 216.85 ± 17.54 mmol g−1 h−1. The sulfate medium also preserved pH stability under high-pressure conditions, ensuring physiological viability essential for M. marburgensis proliferation. Integration of the SBRS-II platform with the MM-CF-S medium signifies a significant advancement toward scalable, efficient biomethanation technologies, highlighting the potential of methanogenic archaea in renewable energy applications and establishing a foundation for further process optimization to meet industrial-scale demands.