Scholarlink

<p>Cholangiocarcinoma (CCA) is an aggressive bile duct malignancy with poor survival rates due to late detection, rapid metastasis, and treatment resistance. Therefore, new therapeutic strategies are needed to improve treatment outcomes. Previous studies have reported that mesenchymal stem cells (MSCs) secrete several soluble factors that modulate intracellular signaling pathways that are critical for the regulation of cancer cell function. The present study aimed to investigate the effects and molecular mechanisms of conditioned media from chorion-derived human MSCs (CH-CM) and placenta-derived human MSCs (PL-CM) on the migration and invasion of human CCA cells. We found that both CH-CM and PL-CM suppress cell migration and invasion in three CCA cell lines (KKU100, KKU213A and KKU213B) by increasing E-cadherin expression and decreasing the expression of several factors involved in the epithelial–mesenchymal transition process, including ZEB1, ZEB2, N-cadherin, vimentin, and MMP-2. The effects of CH-CM and PL-CM were mediated, at least in part, through the suppression of the PI3K/AKT signaling pathway in CCA cells. Our findings suggest that soluble factors derived from CH-MSCs and PL-MSCs could be used in combination with other conventional treatments to diminish the invasiveness of CCA cells, thus improving the therapeutic outcome and increasing survival in CCA patients.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Human chorion and placental mesenchymal stem cells conditioned media suppress cell migration and invasion by inhibiting the PI3K/AKT pathway in cholangiocarcinoma

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月26日

查看全文
  • Tanachapa Jantalika,
  • Sirikul Manochantr,
  • Pakpoom Kheolamai,
  • Duangrat Tantikanlayaporn,
  • Somchai Pinlaor,
  • Weerachai Saijuntha,
  • Luminita Paraoan,
  • Chairat Tantrawatpan

摘要

Cholangiocarcinoma (CCA) is an aggressive bile duct malignancy with poor survival rates due to late detection, rapid metastasis, and treatment resistance. Therefore, new therapeutic strategies are needed to improve treatment outcomes. Previous studies have reported that mesenchymal stem cells (MSCs) secrete several soluble factors that modulate intracellular signaling pathways that are critical for the regulation of cancer cell function. The present study aimed to investigate the effects and molecular mechanisms of conditioned media from chorion-derived human MSCs (CH-CM) and placenta-derived human MSCs (PL-CM) on the migration and invasion of human CCA cells. We found that both CH-CM and PL-CM suppress cell migration and invasion in three CCA cell lines (KKU100, KKU213A and KKU213B) by increasing E-cadherin expression and decreasing the expression of several factors involved in the epithelial–mesenchymal transition process, including ZEB1, ZEB2, N-cadherin, vimentin, and MMP-2. The effects of CH-CM and PL-CM were mediated, at least in part, through the suppression of the PI3K/AKT signaling pathway in CCA cells. Our findings suggest that soluble factors derived from CH-MSCs and PL-MSCs could be used in combination with other conventional treatments to diminish the invasiveness of CCA cells, thus improving the therapeutic outcome and increasing survival in CCA patients.