Scholarlink

<p>Urinary extracellular vesicle (uEV)-derived microRNAs (miRNAs) have emerged as promising noninvasive biomarkers for disease prediction. However, fluctuations in urine concentration throughout the day may influence miRNA abundance and profiles, potentially limiting their clinical utility. To ensure the broader applicability of uEV-derived miRNAs in disease prediction, it is essential that their profiles remain stable regardless of collection time. In this study, we systematically examined factors influencing miRNA abundance in uEVs and assessed the stability of miRNA profiles. We collected serial urine samples from healthy individuals over three days and performed small RNA sequencing to analyze uEV-derived miRNA profiles in relation to various urine concentration parameters. Total miRNA counts were negatively correlated with urine volume and positively correlated with urine concentration indicators, including specific gravity and creatinine levels. miRNA profiles remained stable across different times and days when samples with low miRNA counts were excluded. These findings indicate that urine dilution is a major cause of variation in miRNA abundance. Our results highlight the importance of collecting adequately concentrated samples and provide foundational insights for developing standardized urine collection protocols, thereby facilitating the use of urinary uEV-derived miRNAs as robust and reliable biomarkers for disease prediction.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Robustness of urinary extracellular vesicle-derived MiRNA profiles over multiple days and the impact of urine concentration

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月2日

查看全文
  • Atsushi Satomura,
  • Yoriko Ando,
  • Motoki Mikami,
  • Mika Mizunuma,
  • Yuki Ichikawa

摘要

Urinary extracellular vesicle (uEV)-derived microRNAs (miRNAs) have emerged as promising noninvasive biomarkers for disease prediction. However, fluctuations in urine concentration throughout the day may influence miRNA abundance and profiles, potentially limiting their clinical utility. To ensure the broader applicability of uEV-derived miRNAs in disease prediction, it is essential that their profiles remain stable regardless of collection time. In this study, we systematically examined factors influencing miRNA abundance in uEVs and assessed the stability of miRNA profiles. We collected serial urine samples from healthy individuals over three days and performed small RNA sequencing to analyze uEV-derived miRNA profiles in relation to various urine concentration parameters. Total miRNA counts were negatively correlated with urine volume and positively correlated with urine concentration indicators, including specific gravity and creatinine levels. miRNA profiles remained stable across different times and days when samples with low miRNA counts were excluded. These findings indicate that urine dilution is a major cause of variation in miRNA abundance. Our results highlight the importance of collecting adequately concentrated samples and provide foundational insights for developing standardized urine collection protocols, thereby facilitating the use of urinary uEV-derived miRNAs as robust and reliable biomarkers for disease prediction.