Scholarlink

<p>In this study, three strains of alkaline protease-producing bacteria were isolated from marine seawater collected at a depth of 15 meters off the coast of Ras Sedr at Egypt’s Red Sea. The isolates were morphologically and biochemically characterized, and the most efficient strain was identified via 16S rRNA sequencing, showing 99% similarity to <i>Bacillus amyloliquefaciens</i> MPA 1034. Our newly isolated strain was deposited in the GenBank under the accession number PP034178.1 (<i>Bacillus amyloliquefaciens</i> NRC-IB-11). Six organic waste substrates, namely defatted almond cake, shrimp peel, wheat bran, chicken feathers, defatted flax cake, and defatted soybean cake, were evaluated for enzyme production, with defatted soybean cake yielding the highest activity. Under optimized conditions (10&#xa0;g/100 mL of defatted soybean cake media, 10% v/v inoculum size, pH 10, 35&#xa0;°C, and 160&#xa0;rpm agitation speed), the newly isolated strain produced a maximum of 590 U/mL alkaline protease. This study introduces a novel marine-derived <i>Bacillus</i> strain with promising protease productivity and demonstrates sustainable bioprocessing, utilizing a single-component culture medium to minimize production costs.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimization of protease production by newly isolated Bacillus sp. from the Red Sea using defatted soybean cake

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月1日

查看全文
  • Olfat E. Amin,
  • Ahmed M. Aboul-Enein,
  • Ibrahim S. Abd-Elsalam,
  • Marwa I. Wahba,
  • Yasser S. Helmy

摘要

In this study, three strains of alkaline protease-producing bacteria were isolated from marine seawater collected at a depth of 15 meters off the coast of Ras Sedr at Egypt’s Red Sea. The isolates were morphologically and biochemically characterized, and the most efficient strain was identified via 16S rRNA sequencing, showing 99% similarity to Bacillus amyloliquefaciens MPA 1034. Our newly isolated strain was deposited in the GenBank under the accession number PP034178.1 (Bacillus amyloliquefaciens NRC-IB-11). Six organic waste substrates, namely defatted almond cake, shrimp peel, wheat bran, chicken feathers, defatted flax cake, and defatted soybean cake, were evaluated for enzyme production, with defatted soybean cake yielding the highest activity. Under optimized conditions (10 g/100 mL of defatted soybean cake media, 10% v/v inoculum size, pH 10, 35 °C, and 160 rpm agitation speed), the newly isolated strain produced a maximum of 590 U/mL alkaline protease. This study introduces a novel marine-derived Bacillus strain with promising protease productivity and demonstrates sustainable bioprocessing, utilizing a single-component culture medium to minimize production costs.