Scholarlink

<p>Butylated hydroxytoluene (BHT) is widely employed as an antioxidant in various industries. However, concerns persist regarding its safety and environmental impact, as its toxicological mechanisms remain poorly understood. Zebrafish embryos were exposed to BHT at 3, 5, and 7&#xa0;mg/L to assess developmental toxicity. BHT exposure dose-dependently induced spinal cord malformations concomitant with reduced larval motility. Mechanistically, BHT suppressed Hedgehog signaling, evidenced by dysregulation of Shha, Gli1, and Smo. Notably, pharmacological activation of the Hh pathway by SAG rescued both structural defects and gene expression anomalies, confirming Hh inhibition as the primary toxicity mechanism. These results indicate that BHT inhibits <i>Hh</i> signaling, disrupting floor plate function and leading to spinal curvature.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Butylated hydroxytoluene (BHT) induces zebrafish spinal cord defects and scoliosis by inhibiting the hedgehog pathway

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月7日

查看全文
  • Hao Cheng,
  • Yulian Li,
  • Fasheng Liu,
  • Shanshan Zhu,
  • Li Chen,
  • Huiqiang Lu,
  • Xiaowen Shi,
  • Ling Huang

摘要

Butylated hydroxytoluene (BHT) is widely employed as an antioxidant in various industries. However, concerns persist regarding its safety and environmental impact, as its toxicological mechanisms remain poorly understood. Zebrafish embryos were exposed to BHT at 3, 5, and 7 mg/L to assess developmental toxicity. BHT exposure dose-dependently induced spinal cord malformations concomitant with reduced larval motility. Mechanistically, BHT suppressed Hedgehog signaling, evidenced by dysregulation of Shha, Gli1, and Smo. Notably, pharmacological activation of the Hh pathway by SAG rescued both structural defects and gene expression anomalies, confirming Hh inhibition as the primary toxicity mechanism. These results indicate that BHT inhibits Hh signaling, disrupting floor plate function and leading to spinal curvature.