<p>This study investigated whether a mobile chatbot-delivered intervention for prenatal education could enhance maternal-fetal attachment (MFA) among pregnant women with different levels of spousal support. In a quasi-experimental study conducted in January 2021, sixty volunteers who met all the eligibility criteria were enrolled: legally married, pregnant women aged 19 to 49 years, primigravida women from 21 to 32 weeks of gestation, and those in need of prenatal education. After completing a pre-questionnaire on pregnancy status, perception of prenatal education, and partner support, the women were asked to sequentially perform the given six tasks via a chatbot for two weeks, and then they filled out a post-questionnaire after completing the tasks. Both affective and cognitive-behavioral MFA outcomes were repeatedly measured before and after the intervention. The chatbot intervention enhanced affective MFA by increasing the intensity of prenatal education regardless of spousal support levels (<i>p</i> = .03): (1) low (<i>n</i> = 29; pre vs. post: <i>p</i> &lt; .0042) and (2) high (<i>n</i> = 31; pre vs. post: <i>p</i> &lt; .0042). In terms of cognitive-behavioral MFA, active paternal involvement in the chatbot-assisted baby talk helped women with low spousal support to differentiate themselves from the fetus (<i>p</i> = .02; pre vs. post: <i>p</i> &lt; .0042) and to attribute characteristics and intention to the fetus (<i>p</i> = .011; pre vs. post: <i>p</i> &lt; .0042), eliciting positive affect. This new mobile chatbot successfully served as a guide for pregnant couples starting prenatal education for the first time.</p>