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<p>The interactions of vitamin B6 (pyridoxine hydrochloride) with different concentrations of cationic compounds (cocarboxylase or thiamine pyrophosphate chloride) were investigated in an aqueous solution using UV-VIS absorption spectroscopy. The results depict a hyperchromic shift at 300 ± 1&#xa0;nm, a hypochromic shift at 330 ± 1&#xa0;nm with increasing concentration of carboxylase, and an isosbestic point at 312&#xa0;nm. Hydrophilic-hydrophilic (hydrogen bonding) interaction is the dominating force for the association of two compounds. The binding constant (K<sub>b</sub>) value has been determined by two cases of the Benesi-Hildbrand equation and the concept developed by Hashemi and Sun. The values of K<sub>b</sub> calculated from all three methods were in close agreement. Furthermore, the standard free energy change, ΔG<sup>o</sup> was detected from the computed values of K<sub>b</sub>.</p>

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Spectrophotometric investigation of cocarboxylase interaction with pyridoxine hydrochloride and their interactional properties

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月1日

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  • A. El-Hashani,
  • H. Abuissa,
  • K. Edbey,
  • Pawan Shah,
  • Ajaya Bhattarai

摘要

The interactions of vitamin B6 (pyridoxine hydrochloride) with different concentrations of cationic compounds (cocarboxylase or thiamine pyrophosphate chloride) were investigated in an aqueous solution using UV-VIS absorption spectroscopy. The results depict a hyperchromic shift at 300 ± 1 nm, a hypochromic shift at 330 ± 1 nm with increasing concentration of carboxylase, and an isosbestic point at 312 nm. Hydrophilic-hydrophilic (hydrogen bonding) interaction is the dominating force for the association of two compounds. The binding constant (Kb) value has been determined by two cases of the Benesi-Hildbrand equation and the concept developed by Hashemi and Sun. The values of Kb calculated from all three methods were in close agreement. Furthermore, the standard free energy change, ΔGo was detected from the computed values of Kb.