<p>Shift work presents a variety of health challenges. Despite the acknowledged significance of nutrition in supporting health, there is limited qualitative research on the specific dietary habits and nutritional challenges by healthcare shift workers. The present study aimed to explore challenges in maintaining optimum lifestyle behaviours among healthcare shift workers. Forty healthcare shift workers were interviewed in depth individually. The session was audio recorded and transcribed verbatim, and the interviews were conducted either face-to-face or via video call, depending on the participants’ preferences. Three key themes emerged: food intake, sleep quality, and physical activity. The participants expressed concerns about meal skipping, irregular meal schedules, food accessibility, lack of sleep, and barriers to physical activity. This study highlighted significant challenges faced by healthcare shift workers in maintaining optimal dietary and other lifestyle behaviours, emphasising the interconnectedness of food intake, sleep quality, and physical activity. Addressing such issues is crucial for improving the overall health and well-being of this workforce.</p>