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<p>Extracellular hydrolases associated with nitrogen (N) and phosphorus (P) acquisition are important for soil nutrient cycling. The spatiotemporal patterns of N- and P-hydrolases were rarely studied under N fertilization. It is also unclear whether the N fertilization effects likely vary among different crop species. This study employed a spatially explicit design and clustered soil sampling strategy (288 samples at 0–15&#xa0;cm) in a fertilization experiment with zero, low and high N input (NN, LN and HN: 0, 84, and 168&#xa0;kg N ha<sup>− 1</sup> yr<sup>− 1</sup> urea, respectively) in switchgrass (SG: <i>Panicum virgatum</i> L.) and gamagrass (GG: <i>Tripsacum dactyloides</i> L.) croplands in Middle Tennessee. N-acquisition hydrolases such as leucine aminopeptidase (<i>LAP</i>), β-N-acetylglucosaminidase (<i>NAG</i>), their sum (<i>N</i><sub><i>acq</i></sub>), urease (<i>UREA</i>), and P-acquisition hydrolase acid phosphatase (<i>AP</i>) were quantified. Geostatistical analyses were applied to explore the effects of fertilization and plant type on spatiotemporal variations of N- and P-hydrolases. Results showed large plot-to-plot spatial variation and generally increased variation in soil hydrolyses with N fertilization in both croplands. <i>NAG</i> and <i>N</i><sub><i>acq</i></sub> were significantly higher by 15–32% in GG than in SG soils. Relative to NN, HN significantly increased <i>LAP</i> by 54% in SG soils. <i>LAP</i> appeared to be highly responsive to N fertilization. Overall, this study suggested greater sensitivity and responsiveness of spatiotemporal dynamics to N fertilization in SG cropland. Future studies will examine whether a specific peptidase (i.e., <i>LAP)</i> may facilitate soil C and N sequestration under intensive fertilization in switchgrass soil.</p>

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Effects of nitrogen fertilization and bioenergy crop type on spatial distributions of extracellular hydrolases associated with nitrogen and phosphorus acquisition

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月16日

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  • Jianwei Li,
  • Xuehan Wang,
  • Siyang Jian,
  • Lahiru Gamage,
  • Dafeng Hui,
  • Philip A. Fay

摘要

Extracellular hydrolases associated with nitrogen (N) and phosphorus (P) acquisition are important for soil nutrient cycling. The spatiotemporal patterns of N- and P-hydrolases were rarely studied under N fertilization. It is also unclear whether the N fertilization effects likely vary among different crop species. This study employed a spatially explicit design and clustered soil sampling strategy (288 samples at 0–15 cm) in a fertilization experiment with zero, low and high N input (NN, LN and HN: 0, 84, and 168 kg N ha− 1 yr− 1 urea, respectively) in switchgrass (SG: Panicum virgatum L.) and gamagrass (GG: Tripsacum dactyloides L.) croplands in Middle Tennessee. N-acquisition hydrolases such as leucine aminopeptidase (LAP), β-N-acetylglucosaminidase (NAG), their sum (Nacq), urease (UREA), and P-acquisition hydrolase acid phosphatase (AP) were quantified. Geostatistical analyses were applied to explore the effects of fertilization and plant type on spatiotemporal variations of N- and P-hydrolases. Results showed large plot-to-plot spatial variation and generally increased variation in soil hydrolyses with N fertilization in both croplands. NAG and Nacq were significantly higher by 15–32% in GG than in SG soils. Relative to NN, HN significantly increased LAP by 54% in SG soils. LAP appeared to be highly responsive to N fertilization. Overall, this study suggested greater sensitivity and responsiveness of spatiotemporal dynamics to N fertilization in SG cropland. Future studies will examine whether a specific peptidase (i.e., LAP) may facilitate soil C and N sequestration under intensive fertilization in switchgrass soil.