Scholarlink

<p>The aim of this study is to develop a combined in vitro and in vivo assay for the comprehensive efficacy evaluation of strontium-chelating compounds. The complexation and removal of harmful radioactive strontium (<sup>90</sup>Sr) from the living organism may be necessary in the event of an accidental nuclear disaster or nearby nuclear war event, to prevent its incorporation into bones and thus avoid its long-term harmful health effects. To validate the model, we used a strontium-selective chelator macrocyclic substance (Decorporol) in our measurements, which potentially could be approved as a drug without significant toxic side effects. It has been proven to effectively remove strontium before it is incorporated into bones without significantly affecting calcium homeostasis in the body, and without relevant toxicity or side effects. In this study, we established an extremely sensitive test system that allows for the examination of chelator molecules in preventing the incorporation of non-radioactive SrCl<sub>2</sub> into mineralized extracellular matrix in cell culture. We also optimized an in vivo mouse model suitable to investigate strontium incorporation and the effect of chelators. These assays also provide an opportunity for the safe evaluation of similar compounds.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development and validation of combined in vitro and in vivo assays for evaluating the efficacy of strontium-chelating compounds

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月28日

查看全文
  • Nóra Varga,
  • Viola Pomozi,
  • Eszter Kozák,
  • Zsuzsa Erdei,
  • Szilárd Tóth,
  • Adriána Kutás,
  • Zoltán Mucsi,
  • Zsolt Rapi,
  • Beatrix Kovács,
  • Anett Matuscsák,
  • Olivér Bánhídi,
  • Csaba Váradi,
  • Tamás Rácz,
  • Katalin Német,
  • Béla Viskolcz,
  • Áron Szepesi

摘要

The aim of this study is to develop a combined in vitro and in vivo assay for the comprehensive efficacy evaluation of strontium-chelating compounds. The complexation and removal of harmful radioactive strontium (90Sr) from the living organism may be necessary in the event of an accidental nuclear disaster or nearby nuclear war event, to prevent its incorporation into bones and thus avoid its long-term harmful health effects. To validate the model, we used a strontium-selective chelator macrocyclic substance (Decorporol) in our measurements, which potentially could be approved as a drug without significant toxic side effects. It has been proven to effectively remove strontium before it is incorporated into bones without significantly affecting calcium homeostasis in the body, and without relevant toxicity or side effects. In this study, we established an extremely sensitive test system that allows for the examination of chelator molecules in preventing the incorporation of non-radioactive SrCl2 into mineralized extracellular matrix in cell culture. We also optimized an in vivo mouse model suitable to investigate strontium incorporation and the effect of chelators. These assays also provide an opportunity for the safe evaluation of similar compounds.