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<p>Efficient utilization of cellulose resources is of great significance to environmental protection and to solve the crisis of global energy shortage. In the present work, a polysaccharide (CGP-TS) was successfully produced by <i>Chaetomium globosum</i> CGMCC 6882 from waste tobacco stalk via submerged fermentation. Chemical composition analysis showed that the carbohydrate and protein contents in CGP-TS were 92.55% ± 3.16% and 4.73% ± 1.04%, respectively. Structural characterization indicated that CGP-TS was not pectin and composed of rhamnose, arabinose, galactose, glucose and xylose in a molar ratio of 22.2166: 9.8269: 36.2455: 3.3111: 0.416, and its molecular weight was 613.235&#xa0;kDa. In vitro bioactivity and potential application investigation showed that antioxidant activities of CGP-TS against DPPH, ABTS, hydroxyl and superoxide radicals were positively correlated to its concentration from 0.5&#xa0;mg/mL to 2.5&#xa0;mg/mL. Meanwhile, CGP-TS exhibited immunomodulatory activity via enhancing the phagocytic activity and promoting the cytokines (TNF-α, IL-1β, IL-6 and NO) release of RAW 264.7 macrophages.</p>

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Structural characterization and immunomodulatory activity of a polysaccharide produced by chaetomium globosum CGMCC 6882

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月6日

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  • Shuaiyang Wang,
  • He Chang,
  • Na Li,
  • Danye Zhu,
  • Zichao Wang,
  • Xueyi Qiao,
  • Shaobo Duan

摘要

Efficient utilization of cellulose resources is of great significance to environmental protection and to solve the crisis of global energy shortage. In the present work, a polysaccharide (CGP-TS) was successfully produced by Chaetomium globosum CGMCC 6882 from waste tobacco stalk via submerged fermentation. Chemical composition analysis showed that the carbohydrate and protein contents in CGP-TS were 92.55% ± 3.16% and 4.73% ± 1.04%, respectively. Structural characterization indicated that CGP-TS was not pectin and composed of rhamnose, arabinose, galactose, glucose and xylose in a molar ratio of 22.2166: 9.8269: 36.2455: 3.3111: 0.416, and its molecular weight was 613.235 kDa. In vitro bioactivity and potential application investigation showed that antioxidant activities of CGP-TS against DPPH, ABTS, hydroxyl and superoxide radicals were positively correlated to its concentration from 0.5 mg/mL to 2.5 mg/mL. Meanwhile, CGP-TS exhibited immunomodulatory activity via enhancing the phagocytic activity and promoting the cytokines (TNF-α, IL-1β, IL-6 and NO) release of RAW 264.7 macrophages.