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<p>Patients with ulcerative colitis (UC) have a significantly impaired intestinal barrier. Hydrogen Sulfide (H<sub>2</sub>S) is a gaseous mediator that makes notable contributions in a variety of diseases, such as reducing inflammatory response in colitis. The experimental content includes the establishment of a mouse DSS-induced colitis mouse model, mouse colon epithelial organoids culture, H&amp;E staining and mass spectrometry analysis. We recognized that exogenous H<sub>2</sub>S donor-GYY4137 significantly alleviated the symptoms in UC mice models and maintained Minichromosome Maintenance Complex Component 2 (MCM2) expression. CBS knockdown reduced the expression of sulfhydrated Ribosomal protein S20 (RPS20-ssh) and MCM2 in the mouse colon. Cell experiments indicated that the expression of RPS20-ssh, rather than total expression of RPS20, is responsible.</p><p>Our investigation indicated that CBS-H<sub>2</sub>S axis increases the sulfhydration level of RPS20, leading to enhanced binding between RPS20 and MCM2 mRNA, thereby promoting intestinal epithelial proliferation. This may provide a novel therapeutic strategy for the clinical treatment of colitis.</p>

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Hydrogen sulfide preserves intestinal barrier repair function through sulfhydration of RPS20 in experimental colitis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月21日

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  • Xuan He,
  • Jichang Li,
  • Zhihao Huang,
  • Baoshuai Xue,
  • Jing Zhu,
  • Meiyi You,
  • Xiaoyun Liu,
  • Xin Wang,
  • Yucun Liu,
  • Shanwen Chen,
  • Pengyuan Wang

摘要

Patients with ulcerative colitis (UC) have a significantly impaired intestinal barrier. Hydrogen Sulfide (H2S) is a gaseous mediator that makes notable contributions in a variety of diseases, such as reducing inflammatory response in colitis. The experimental content includes the establishment of a mouse DSS-induced colitis mouse model, mouse colon epithelial organoids culture, H&E staining and mass spectrometry analysis. We recognized that exogenous H2S donor-GYY4137 significantly alleviated the symptoms in UC mice models and maintained Minichromosome Maintenance Complex Component 2 (MCM2) expression. CBS knockdown reduced the expression of sulfhydrated Ribosomal protein S20 (RPS20-ssh) and MCM2 in the mouse colon. Cell experiments indicated that the expression of RPS20-ssh, rather than total expression of RPS20, is responsible.

Our investigation indicated that CBS-H2S axis increases the sulfhydration level of RPS20, leading to enhanced binding between RPS20 and MCM2 mRNA, thereby promoting intestinal epithelial proliferation. This may provide a novel therapeutic strategy for the clinical treatment of colitis.