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<p>Vaccine approaches for controlling <i>Fasciola hepatica</i> present a promising avenue, particularly considering increasing resistance to anthelmintic treatments and concerns over chemical residues. Targeting vaccine candidates that are expressed and secreted during the early infective stage of <i>F. hepatica</i> could offer an effective alternative. This approach aims to inhibit the invasion and migration of juvenile parasites, which have not yet fully developed their immune evasion mechanisms, thereby preventing parasite establishment and development in the host. In this study, we evaluated the host immune response and the protective efficacy of a vaccine cocktail comprising four antigens -KTSPIDP, VGHC1, CRTA, and CAL- in sheep infected with <i>F. hepatica</i>. These parasitic antigens were selected based on a proteomic analysis coupled with an “in vitro” interaction model between newly excysted juvenile worms and mouse intestinal epithelial cell cultures. Despite inducing a strong IgG1 response, vaccination did not reduce liver fluke burden nor faecal egg counts. However, it reduced liver pathology caused by the parasite. Our findings highlight the need for further research into early-stage interactions between <i>F. hepatica</i> and the host. Understanding these interactions could facilitate the progress of vaccines capable of disrupting parasite development and transmission in livestock, potentially reducing the economic and health impacts associated with fasciolosis.</p>

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Evaluation of a novel vaccine candidate derived from newly excysted juveniles of Fasciola hepatica in sheep

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月3日

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  • Elora Valderas-García,
  • Rafael Zafra,
  • Pablo J. Rufino-Moya,
  • F. Javier Martínez-Moreno,
  • María T. Ruiz‑Campillo,
  • Verónica Molina‑Hernández,
  • Javier González-Miguel,
  • Mar Siles-Lucas,
  • José Pérez,
  • Álvaro Martínez-Moreno,
  • Leandro Buffoni

摘要

Vaccine approaches for controlling Fasciola hepatica present a promising avenue, particularly considering increasing resistance to anthelmintic treatments and concerns over chemical residues. Targeting vaccine candidates that are expressed and secreted during the early infective stage of F. hepatica could offer an effective alternative. This approach aims to inhibit the invasion and migration of juvenile parasites, which have not yet fully developed their immune evasion mechanisms, thereby preventing parasite establishment and development in the host. In this study, we evaluated the host immune response and the protective efficacy of a vaccine cocktail comprising four antigens -KTSPIDP, VGHC1, CRTA, and CAL- in sheep infected with F. hepatica. These parasitic antigens were selected based on a proteomic analysis coupled with an “in vitro” interaction model between newly excysted juvenile worms and mouse intestinal epithelial cell cultures. Despite inducing a strong IgG1 response, vaccination did not reduce liver fluke burden nor faecal egg counts. However, it reduced liver pathology caused by the parasite. Our findings highlight the need for further research into early-stage interactions between F. hepatica and the host. Understanding these interactions could facilitate the progress of vaccines capable of disrupting parasite development and transmission in livestock, potentially reducing the economic and health impacts associated with fasciolosis.