Scholarlink

<p>The pathogenic oomycete <i>Pythium insidiosum</i> causes a fatal infectious illness known as pythiosis, impacting humans and certain animals in numerous countries in the tropics and subtropics. Delayed diagnosis is a primary factor contributing to the heightened morbidity and mortality associated with the disease. Several new serodiagnostic methods have been developed to improve the identification of pythiosis. However, these assays provide only indirect evidence of pythiosis and are not readily available in the commercial market. Here, we have developed an affordable point-of-care test (POCT) kit based on an immunochromatographic assay to detect <i>P. insidiosum</i> antigens directly. Our recent findings reveal that the lateral flow sandwich immunological testing cassette can accurately identify vascular pythiosis antigens using a small volume of patient’s plasma, accomplishing diagnostic efficiency, with a limit of detection (LOD) of 8 ng/mL. This prototype cartridge represents a significant stride toward advancing enriched POCT for pythiosis serodiagnosis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development of lateral flow immunochromatographic assay with Anti-Pythium insidiosum antibodies for point-of-care testing of vascular pythiosis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月4日

查看全文
  • Panwad Tongchai,
  • Ati Burassakarn,
  • Nattapong Langsiri,
  • Navaporn Worasilchai,
  • Ariya Chindamporn

摘要

The pathogenic oomycete Pythium insidiosum causes a fatal infectious illness known as pythiosis, impacting humans and certain animals in numerous countries in the tropics and subtropics. Delayed diagnosis is a primary factor contributing to the heightened morbidity and mortality associated with the disease. Several new serodiagnostic methods have been developed to improve the identification of pythiosis. However, these assays provide only indirect evidence of pythiosis and are not readily available in the commercial market. Here, we have developed an affordable point-of-care test (POCT) kit based on an immunochromatographic assay to detect P. insidiosum antigens directly. Our recent findings reveal that the lateral flow sandwich immunological testing cassette can accurately identify vascular pythiosis antigens using a small volume of patient’s plasma, accomplishing diagnostic efficiency, with a limit of detection (LOD) of 8 ng/mL. This prototype cartridge represents a significant stride toward advancing enriched POCT for pythiosis serodiagnosis.