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<p>A comprehensive polyphasic characterization has validated the unique taxonomic position of a novel bacterium, strain EMC7<sup>T</sup>, isolated from the worm castings of earthworm, <i>Eisenia fetida</i>, collected from the Centre for Floriculture and Agri-Business Management (COFAM), NBU (26.7072° N, 88.3554° E). Whole-genome sequence of this Gram-stain-negative, facultatively anaerobic, motile, rod-shaped bacterium showed maximum sequence homology with <i>Leclercia adecarboxylata</i> NBRC 102595<sup>T</sup>, placing it within the genus <i>Leclercia</i>. The genome of EMC7<sup>T</sup> is 5.03 Mbp with a G + C content of 56.3&#xa0;mol%. Phylogenetic analyses established its distinctiveness from <i>Leclercia adecarboxylata</i> and <i>Leclercia tamurae</i>. DNA-DNA hybridization (dDDH) value was 23.6%, and the average nucleotide identity (ANI) was 82.1%, both below the thresholds for prokaryotic species differentiation. Predominant fatty acids were C<sub>16:0</sub> (29.53%), summed feature 3 (C<sub>16:1</sub>ω7c/C<sub>16:1</sub>ω6c, 16.51%), and C<sub>18:1</sub>ω7c (10.90%). Notably, EMC7<sup>T</sup> exhibited urease activity and could metabolize 3-nitropropionic acid (3-NPA), glycerol, tellurite, selenate, and selenite, suggesting potential bioremediation applications. Biochemical tests, phenotypic traits, genotypic data, and physiological properties cumulatively differentiated EMC7<sup>T</sup> from its closest relatives. Based on chemotaxonomic, phenotypic, genomic, and phylogenetic evidence, strain EMC7<sup>T</sup> represents a novel bacterial species of the genus <i>Leclercia</i>, for which the name <i>Leclercia barmai</i> sp. nov. (type strain EMC7<sup>T</sup> = MCC 5183<sup>T</sup> = JCM 36544<sup>T</sup>) is proposed.</p>

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Leclercia barmai sp. nov., isolated from worm castings of Eisenia fetida, is a urease-positive, 3-nitropropionic acid and glycerol-consuming bacterium

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  • 发表日期：

    2025年2月15日

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  • Partha Barman,
  • Shilpa Sinha,
  • Ranadhir Chakraborty

摘要

A comprehensive polyphasic characterization has validated the unique taxonomic position of a novel bacterium, strain EMC7T, isolated from the worm castings of earthworm, Eisenia fetida, collected from the Centre for Floriculture and Agri-Business Management (COFAM), NBU (26.7072° N, 88.3554° E). Whole-genome sequence of this Gram-stain-negative, facultatively anaerobic, motile, rod-shaped bacterium showed maximum sequence homology with Leclercia adecarboxylata NBRC 102595T, placing it within the genus Leclercia. The genome of EMC7T is 5.03 Mbp with a G + C content of 56.3 mol%. Phylogenetic analyses established its distinctiveness from Leclercia adecarboxylata and Leclercia tamurae. DNA-DNA hybridization (dDDH) value was 23.6%, and the average nucleotide identity (ANI) was 82.1%, both below the thresholds for prokaryotic species differentiation. Predominant fatty acids were C16:0 (29.53%), summed feature 3 (C16:1ω7c/C16:1ω6c, 16.51%), and C18:1ω7c (10.90%). Notably, EMC7T exhibited urease activity and could metabolize 3-nitropropionic acid (3-NPA), glycerol, tellurite, selenate, and selenite, suggesting potential bioremediation applications. Biochemical tests, phenotypic traits, genotypic data, and physiological properties cumulatively differentiated EMC7T from its closest relatives. Based on chemotaxonomic, phenotypic, genomic, and phylogenetic evidence, strain EMC7T represents a novel bacterial species of the genus Leclercia, for which the name Leclercia barmai sp. nov. (type strain EMC7T = MCC 5183T = JCM 36544T) is proposed.