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<p>In this study, the endophytic fungus <i>Coniothyrium chaingmaiense</i>-KUMBMDBT-25 was isolated from the healthy stem of <i>Euphorbia tirucalli</i>, mass cultivated by submerged fermentation, and extracted using ethyl acetate as a solvent. The extract was subjected to GC-MS analysis. The synthesized Con-AgNPs were characterized through various bioanalytical methods. The synthesis was confirmed by Bio- spectrophotometry, which showed an absorption peak at 404&#xa0;nm. FTIR analysis verified the reduction and capping of Con-AgNPs, displaying peaks corresponding to various functional groups. SEM-EDAX and HR-TEM examinations revealed that the Con-AgNPs were spherical, and EDAX analysis confirmed the presence of silver atoms at 3&#xa0;keV. XRD studies revealed the crystalline structure of Con-AgNPs. DLS and Zeta potential tests determined the size and stability of the synthesized Con-AgNPs, which were 65.81&#xa0;nm. The Con-AgNPs demonstrated strong antibacterial activity against <i>P. aeruginosa</i> (14.06 ± 0.11&#xa0;mm, 10&#xa0;mg/mL) and effective antifungal activity against <i>A. flavus</i> (13.03 ± 0.05&#xa0;mm, 10&#xa0;mg/mL). Con-AgNPs exhibited notable biological attributes, including a cytotoxic effect of up to 38.82% and 19.15% at 200&#xa0;µg/mL in an MTT assay measuring cell viability. Additionally, the nanoparticles demonstrated significant anti-inflammatory effects in both in vitro and in vivo studies, validating the biological and pharmacological potential of Con-AgNPs.</p>

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Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月3日

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  • Manjunatha Dadayya,
  • Megha Gowri Thippeswamy,
  • Nagaraju Shivaiah,
  • Thoyajakshi Ramasamudra Siddaraju,
  • Prakash Jayaramaiah,
  • Sowmya Hirakannavar Veeranna,
  • Thippeswamy Basaiah,
  • Shridhar N Mathad,
  • Ravikumar Hemagiri Gowda,
  • Sachin Naik,
  • Abdulaziz Abdullah AI Kheraif,
  • Sajith Vellappally

摘要

In this study, the endophytic fungus Coniothyrium chaingmaiense-KUMBMDBT-25 was isolated from the healthy stem of Euphorbia tirucalli, mass cultivated by submerged fermentation, and extracted using ethyl acetate as a solvent. The extract was subjected to GC-MS analysis. The synthesized Con-AgNPs were characterized through various bioanalytical methods. The synthesis was confirmed by Bio- spectrophotometry, which showed an absorption peak at 404 nm. FTIR analysis verified the reduction and capping of Con-AgNPs, displaying peaks corresponding to various functional groups. SEM-EDAX and HR-TEM examinations revealed that the Con-AgNPs were spherical, and EDAX analysis confirmed the presence of silver atoms at 3 keV. XRD studies revealed the crystalline structure of Con-AgNPs. DLS and Zeta potential tests determined the size and stability of the synthesized Con-AgNPs, which were 65.81 nm. The Con-AgNPs demonstrated strong antibacterial activity against P. aeruginosa (14.06 ± 0.11 mm, 10 mg/mL) and effective antifungal activity against A. flavus (13.03 ± 0.05 mm, 10 mg/mL). Con-AgNPs exhibited notable biological attributes, including a cytotoxic effect of up to 38.82% and 19.15% at 200 µg/mL in an MTT assay measuring cell viability. Additionally, the nanoparticles demonstrated significant anti-inflammatory effects in both in vitro and in vivo studies, validating the biological and pharmacological potential of Con-AgNPs.