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<p>The tree shrew (<i>Tupaia belangeri chinensis</i>), as a species closely related to primates, exhibits significant seasonal reproductive traits in adult males, including cyclical changes in testicular morphology and spermatogenesis between the reproductive and non-reproductive seasons. However, comprehensive and systematic data resources on the molecular expression features associated with this physiological phenomenon are still lacking. In this study, we utilized the Illumina high-throughput sequencing platform to perform RNA-seq and small RNA-seq on testicular, caput epididymis, and caudal epididymis tissues collected from male tree shrews during both reproductive and non-reproductive seasons. These analyses were performed to generate a paired mRNA and small RNA dataset covering multiple tissues and seasonal states. RNA-seq analysis identified 21,299 expressed genes across three reproductive tissues and two seasonal states. Small RNA-seq analysis identified 353 known miRNAs, 263 novel miRNAs, 59,590 piRNAs, and 139 piRNA clusters across all libraries. Differential expression results for all RNA species, functional enrichment analyses, co-expression network outputs, and predicted regulatory network data are available in the accompanying data repository. This multi-tissue, multi-season dataset provides a systematic and reusable transcriptomic resource for investigating gene expression and small RNA regulation associated with seasonal reproduction in male tree shrews, and may serve as a reference for studies of reproductive biology in non-model mammals.</p>

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mRNA and small RNA landscapes of tree shrew(Tupaia belangeri chinensis) testes and epididymides during reproductive and non-reproductive seasons

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月30日

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  • Xu Dai,
  • Daohao Xie,
  • Minghua Yang,
  • Yahui Li

摘要

The tree shrew (Tupaia belangeri chinensis), as a species closely related to primates, exhibits significant seasonal reproductive traits in adult males, including cyclical changes in testicular morphology and spermatogenesis between the reproductive and non-reproductive seasons. However, comprehensive and systematic data resources on the molecular expression features associated with this physiological phenomenon are still lacking. In this study, we utilized the Illumina high-throughput sequencing platform to perform RNA-seq and small RNA-seq on testicular, caput epididymis, and caudal epididymis tissues collected from male tree shrews during both reproductive and non-reproductive seasons. These analyses were performed to generate a paired mRNA and small RNA dataset covering multiple tissues and seasonal states. RNA-seq analysis identified 21,299 expressed genes across three reproductive tissues and two seasonal states. Small RNA-seq analysis identified 353 known miRNAs, 263 novel miRNAs, 59,590 piRNAs, and 139 piRNA clusters across all libraries. Differential expression results for all RNA species, functional enrichment analyses, co-expression network outputs, and predicted regulatory network data are available in the accompanying data repository. This multi-tissue, multi-season dataset provides a systematic and reusable transcriptomic resource for investigating gene expression and small RNA regulation associated with seasonal reproduction in male tree shrews, and may serve as a reference for studies of reproductive biology in non-model mammals.