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<p>Dermal papilla cells (DPCs) are specialized mesenchymal cells at the base of the hair follicle that function as pivotal signalling centre essential for follicle morphogenesis and regeneration. In cashmere goats, primary hair follicles (PHFs) generate coarse guard hair, whereas secondary hair follicles (SHFs) produce the fine cashmere fibre. To investigate the molecular mechanisms underlying DPC-mediated regulation of fibre production, we isolated highly pure and viable DPCs from PHFs and anatomically paired SHFs at early anagen and anagen phases in both male and female goats, following a laboratory-validated dermal papilla isolation protocol. Transcriptome profiling revealed distinct molecular signatures associated with growth phase, follicle type, and, unexpectedly, sex, highlighting a previously underexplored dimension of DPCs. Across these comparisons, 3,857 differentially expressed genes were identified, with representative results confirmed by real-time PCR. Collectively, these datasets provide novel comprehensive transcriptomic characterization of DPC heterogeneity in cashmere growth, uncovering growth-phase, follicle-type, and sex-associated regulatory features. This resource establishes a foundation for mechanistic studies of hair follicle and supports future strategies to enhance cashmere yield and quality.</p>

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Transcriptomic profiling of dermal papilla cells across growth phases, hair follicle types, and sexes in cashmere goats

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月23日

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  • Yanlei Liu,
  • Hongji Yu,
  • Hongxia Li,
  • Yiping Wei,
  • Minglin Wang,
  • Xinyue Liang,
  • Xinghui Zhang,
  • Bohan Liu,
  • Junpeng Zhang,
  • Shuyi Zhang,
  • Huiling Xue

摘要

Dermal papilla cells (DPCs) are specialized mesenchymal cells at the base of the hair follicle that function as pivotal signalling centre essential for follicle morphogenesis and regeneration. In cashmere goats, primary hair follicles (PHFs) generate coarse guard hair, whereas secondary hair follicles (SHFs) produce the fine cashmere fibre. To investigate the molecular mechanisms underlying DPC-mediated regulation of fibre production, we isolated highly pure and viable DPCs from PHFs and anatomically paired SHFs at early anagen and anagen phases in both male and female goats, following a laboratory-validated dermal papilla isolation protocol. Transcriptome profiling revealed distinct molecular signatures associated with growth phase, follicle type, and, unexpectedly, sex, highlighting a previously underexplored dimension of DPCs. Across these comparisons, 3,857 differentially expressed genes were identified, with representative results confirmed by real-time PCR. Collectively, these datasets provide novel comprehensive transcriptomic characterization of DPC heterogeneity in cashmere growth, uncovering growth-phase, follicle-type, and sex-associated regulatory features. This resource establishes a foundation for mechanistic studies of hair follicle and supports future strategies to enhance cashmere yield and quality.