<p><i>Cremastra appendiculata</i> (D. Don) Makino, an endangered terrestrial orchid with significant medicinal value, lacks comprehensive genomic resources to elucidate its evolution and conservation genetics. Here, we present a chromosome-level genome assembly for <i>C. appendiculata</i>, generated by integrating PacBio circular consensus sequencing and Hi-C technologies. The final genome assembly totals 2.40 Gb, achieving contig and scaffold N50 values of 27.47 Mb and 98.06 Mb, respectively. Hi-C scaffolding anchored 98.66% of the assembly to 24 chromosomes, with repetitive elements comprising 83.85% of the genome and a total of 31,703 protein-coding genes predicted, of which 29,094 (91.77%) were functionally annotated. This high-quality, chromosome-level reference genome provides a foundational resource for understanding the population genetic structure, adaptive evolution and speciation mechanisms of <i>C. appendiculata</i>, thereby offering valuable insights into its evolutionary history and conservation.</p>

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A high-quality chromosome-level genome assembly and annotation of the medicinal orchid Cremastra appendiculata (Orchidaceae)

  • Yongchao Tang,
  • Bo Xiao,
  • Ruimin Yu,
  • Shubin Dong,
  • Xiujin Qi,
  • Yibo Luo,
  • Xiaohong Zhao,
  • Jin Cheng

摘要

Cremastra appendiculata (D. Don) Makino, an endangered terrestrial orchid with significant medicinal value, lacks comprehensive genomic resources to elucidate its evolution and conservation genetics. Here, we present a chromosome-level genome assembly for C. appendiculata, generated by integrating PacBio circular consensus sequencing and Hi-C technologies. The final genome assembly totals 2.40 Gb, achieving contig and scaffold N50 values of 27.47 Mb and 98.06 Mb, respectively. Hi-C scaffolding anchored 98.66% of the assembly to 24 chromosomes, with repetitive elements comprising 83.85% of the genome and a total of 31,703 protein-coding genes predicted, of which 29,094 (91.77%) were functionally annotated. This high-quality, chromosome-level reference genome provides a foundational resource for understanding the population genetic structure, adaptive evolution and speciation mechanisms of C. appendiculata, thereby offering valuable insights into its evolutionary history and conservation.