Scholarlink

<p><i>Maccullochella peelii</i>, commonly known as Murray cod, is an endemic economic fish from the Australian freshwater systems. In order to provide high-quality reference genome for its genomic studies, we assemble and annotate the first near telomere-to-telomere genome of this species which spans 762.84 Mb with a contig N50 of 31.21 Mb, 99.02% sequences were positioned to 24 chromosomes by Hi-C assembly. After gap filling using ONT ultra-long reads, yielding 22 gapless chromosomes. Based on homologous predictions and RNA-sequencing, 27,380 protein-coding genes were identified, and the functions were predicted for 24,998 (91.3%) of them. Moreover, assembled genome was evaluated as 99.3% single-copy BUSCOs completeness. This work could provide a foundation and high-quality genomic resources for the future research on molecular breeding and population genetics of this species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The near telomere-to-telomere genome of the Murray cod (Maccullochella peelii)

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月25日

查看全文
  • Li Li,
  • Youhong Wang,
  • Dong Liu,
  • Ying Fan,
  • Shuxian Wang,
  • Xiaolu Wang,
  • Chunlei Gai,
  • Haibin Ye

摘要

Maccullochella peelii, commonly known as Murray cod, is an endemic economic fish from the Australian freshwater systems. In order to provide high-quality reference genome for its genomic studies, we assemble and annotate the first near telomere-to-telomere genome of this species which spans 762.84 Mb with a contig N50 of 31.21 Mb, 99.02% sequences were positioned to 24 chromosomes by Hi-C assembly. After gap filling using ONT ultra-long reads, yielding 22 gapless chromosomes. Based on homologous predictions and RNA-sequencing, 27,380 protein-coding genes were identified, and the functions were predicted for 24,998 (91.3%) of them. Moreover, assembled genome was evaluated as 99.3% single-copy BUSCOs completeness. This work could provide a foundation and high-quality genomic resources for the future research on molecular breeding and population genetics of this species.