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<p>Blackleg disease, caused by the ascomycete fungus <i>Plenodomus lingam</i> (syn.: <i>Leptosphaeria maculans</i>), is the most devastating disease of oilseed rape worldwide. As a model organism for studying plant-fungus interactions, <i>P. lingam</i> has been extensively studied, yet telomere-to-telomere (T2T) genome assemblies have been lacking. Here, we report a high-quality T2T gapless genome assembly of <i>P. lingam</i> ‘brassicae’ strain 479-2, generated using MGI short reads, PacBio/Nanopore long reads, and Hi-C data. The assembly comprises 19 nuclear chromosomes totaling 40.74 Mb, each capped by telomeric repeats (CCCTAA/TTAGGG), and a complete circular mitochondrial genome of 169,227 bp. Key quality metrics include: 46.08% GC content, 30.09% repeat content, 98.5% complete BUSCOs (fungi_odb10), a consensus quality score of 45.86, and a low error rate of 2.59 × 10<sup>−5</sup>. Functional annotation predicted 9,818 protein-coding genes, including 807 cytochrome P450s, 464 carbohydrate-active enzymes, 808 secreted proteins, and 287 candidate effectors. This T2T assembly provides a gold-standard genomic resource for studies of pathogenicity, effector evolution, and host adaptation in <i>P. lingam</i>, and will advance research on plant-pathogen co-evolution.</p>

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First telomere-to-telomere gapless genome assembly of the blackleg fungus Plenodomus lingam

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月21日

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  • Tao Luo,
  • Mingde Wu,
  • Jing Zhang,
  • Guoqing Li,
  • Long Yang

摘要

Blackleg disease, caused by the ascomycete fungus Plenodomus lingam (syn.: Leptosphaeria maculans), is the most devastating disease of oilseed rape worldwide. As a model organism for studying plant-fungus interactions, P. lingam has been extensively studied, yet telomere-to-telomere (T2T) genome assemblies have been lacking. Here, we report a high-quality T2T gapless genome assembly of P. lingam ‘brassicae’ strain 479-2, generated using MGI short reads, PacBio/Nanopore long reads, and Hi-C data. The assembly comprises 19 nuclear chromosomes totaling 40.74 Mb, each capped by telomeric repeats (CCCTAA/TTAGGG), and a complete circular mitochondrial genome of 169,227 bp. Key quality metrics include: 46.08% GC content, 30.09% repeat content, 98.5% complete BUSCOs (fungi_odb10), a consensus quality score of 45.86, and a low error rate of 2.59 × 10−5. Functional annotation predicted 9,818 protein-coding genes, including 807 cytochrome P450s, 464 carbohydrate-active enzymes, 808 secreted proteins, and 287 candidate effectors. This T2T assembly provides a gold-standard genomic resource for studies of pathogenicity, effector evolution, and host adaptation in P. lingam, and will advance research on plant-pathogen co-evolution.