<p>The Carajás morning glory (<i>Ipomoea cavalcantei</i> D.F. Austin) is a plant species endemic to ironstone outcrops in the eastern Amazon, Brazil. Here, we present a chromosome-level reference genome combining PacBio HiFi and HiC sequencing data for this species. Our high-quality assembly of 1.02 Gb consisted of 314 contigs anchored to 15 chromosomes, with a contig N50 of 15.9 Mb and a scaffold N50 of 63.1 Mb. Benchmarking Universal Single-Copy Orthologue (BUSCO) analysis demonstrated that the genome assembly achieved high completeness, with a score of 94.92%. We obtained 25,335 unique protein-coding loci. Repetitive sequences constituted 73.96% of the total genome length. This phased chromosome-level genome paves the way for investigations into the species’ evolutionary history, molecular mechanisms associated with adaptation to its specialized niche, and to subsidize conservation efforts aimed at preserving this unique component of the Amazonian biodiversity.</p>

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Diploid chromosome-level genome assembly of the Amazonian endemic morning glory Ipomoea cavalcantei D.F. Austin

  • Lucas Eduardo Costa Canesin,
  • Sibelle Torres Villaça,
  • Leandro Magalhães,
  • Carlos Eduardo de Araújo Batista,
  • Jacqueline de Souza Lima,
  • Alexandre Aleixo,
  • Valéria da Cunha Tavares,
  • Amanda Ferreira Vidal,
  • Carolina da Silva Carvalho

摘要

The Carajás morning glory (Ipomoea cavalcantei D.F. Austin) is a plant species endemic to ironstone outcrops in the eastern Amazon, Brazil. Here, we present a chromosome-level reference genome combining PacBio HiFi and HiC sequencing data for this species. Our high-quality assembly of 1.02 Gb consisted of 314 contigs anchored to 15 chromosomes, with a contig N50 of 15.9 Mb and a scaffold N50 of 63.1 Mb. Benchmarking Universal Single-Copy Orthologue (BUSCO) analysis demonstrated that the genome assembly achieved high completeness, with a score of 94.92%. We obtained 25,335 unique protein-coding loci. Repetitive sequences constituted 73.96% of the total genome length. This phased chromosome-level genome paves the way for investigations into the species’ evolutionary history, molecular mechanisms associated with adaptation to its specialized niche, and to subsidize conservation efforts aimed at preserving this unique component of the Amazonian biodiversity.