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<p>We report the first chromosome-level genome assembly of the critically endangered dwarf cattail, <i>Typha minima</i>, a wetland species of ecological and medicinal importance. Utilizing PacBio HiFi long-read sequencing and Hi-C scaffolding technologies, we generated a high-quality 324.66 Mb genome, anchored onto 30 pseudochromosomes. The assembly demonstrates exceptional continuity, with contig and scaffold N50 values of 10.84 Mb and 10.90 Mb, respectively, and a near-complete chromosomal anchoring rate of 99.65%. It exhibits outstanding completeness, as reflected by a BUSCO score of 99.2%, and contains 33.20% repetitive sequences. We annotated 34,541 protein-coding genes, with 96.42% receiving functional assignments. The assembly also includes annotations for non-coding RNAs, comprising 1,261 rRNAs, 230 miRNAs, and 467 tRNAs. Integrated orthology analysis identified 10,055 consensus orthologs across five functional databases. This high-quality genomic resource provides a foundation for advancing studies in evolutionary adaptation and conservation genomics of this endangered wetland plant.</p>

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Chromosome-level genome assembly of the dwarf cattail Typha minima

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月10日

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  • Junshuai Du,
  • Lei Huang,
  • Xinwei Xu

摘要

We report the first chromosome-level genome assembly of the critically endangered dwarf cattail, Typha minima, a wetland species of ecological and medicinal importance. Utilizing PacBio HiFi long-read sequencing and Hi-C scaffolding technologies, we generated a high-quality 324.66 Mb genome, anchored onto 30 pseudochromosomes. The assembly demonstrates exceptional continuity, with contig and scaffold N50 values of 10.84 Mb and 10.90 Mb, respectively, and a near-complete chromosomal anchoring rate of 99.65%. It exhibits outstanding completeness, as reflected by a BUSCO score of 99.2%, and contains 33.20% repetitive sequences. We annotated 34,541 protein-coding genes, with 96.42% receiving functional assignments. The assembly also includes annotations for non-coding RNAs, comprising 1,261 rRNAs, 230 miRNAs, and 467 tRNAs. Integrated orthology analysis identified 10,055 consensus orthologs across five functional databases. This high-quality genomic resource provides a foundation for advancing studies in evolutionary adaptation and conservation genomics of this endangered wetland plant.