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<p><i>Pulsatilla chinensis</i> (Bunge) Regel, a medicinal species in the genus <i>Pulsatilla</i> (Ranunculaceae), exhibits significant anti-inflammatory activity due to its abundant triterpenoid saponins, enhancing its medicinal value. All 48 known <i>Pulsatilla</i> species lack whole genome sequencing data, hindering research on the utilization of genetic resources in the genus and investigations into their biosynthetic mechanisms. Here, we present the first chromosome-level genome assembly of <i>P. chinensis</i>. The assembly spans 6,588.76 Mb with a contig N50 of 4.28 Mb. Using PacBio sequencing and Hi-C scaffolding, 99.32% of sequences were anchored onto eight chromosomes. We annotated 41,786 protein-coding genes through <i>ab initio</i> prediction, homology alignment, and transcriptome evidence, with 98.77% functionally annotated via NR, GO, and KEGG databases. This assembly not only facilitates studies on the molecular mechanisms and biosynthesis of bioactive compounds in <i>P. chinensis</i>, but also supports the further enrichment and utilization of germplasm resources across the Ranunculaceae.</p>

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A high-quality chromosome-level reference genome of Pulsatilla chinensis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月24日

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  • Ting Wang,
  • Chenhao Zhang,
  • Xiao Ma,
  • Yanhong Fu,
  • Yinglong Xiang,
  • Chengyan Wu,
  • Shaoqin Shen,
  • Zhuo Liu,
  • Kejun Deng,
  • Hao Lin,
  • Xiaoming Song

摘要

Pulsatilla chinensis (Bunge) Regel, a medicinal species in the genus Pulsatilla (Ranunculaceae), exhibits significant anti-inflammatory activity due to its abundant triterpenoid saponins, enhancing its medicinal value. All 48 known Pulsatilla species lack whole genome sequencing data, hindering research on the utilization of genetic resources in the genus and investigations into their biosynthetic mechanisms. Here, we present the first chromosome-level genome assembly of P. chinensis. The assembly spans 6,588.76 Mb with a contig N50 of 4.28 Mb. Using PacBio sequencing and Hi-C scaffolding, 99.32% of sequences were anchored onto eight chromosomes. We annotated 41,786 protein-coding genes through ab initio prediction, homology alignment, and transcriptome evidence, with 98.77% functionally annotated via NR, GO, and KEGG databases. This assembly not only facilitates studies on the molecular mechanisms and biosynthesis of bioactive compounds in P. chinensis, but also supports the further enrichment and utilization of germplasm resources across the Ranunculaceae.