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<p><i>Magnolia zenii</i> (Magnoliaceae) is an extremely endangered tree species endemic to eastern China, has only 115 individuals remaining in the wild. By integrating PacBio HiFi (49×), Oxford Nanopore (ONT; 101×) long-read sequencing, and Hi-C chromatin interaction data, we achieved a high-quality chromosome-level genome with scaffold N50 of 86 Mb, covering 99.97% of the estimated genome size. The genome annotation predicted 74,782 protein-coding genes and revealed a repetitive sequence content of 72.87%, dominated by long terminal repeat (LTR) retrotransposons (53.59%). The mitochondrial (915,126 bp) and chloroplast (160,079 bp) genomes were fully characterized. BUSCO analysis confirmed 99.4% genome completeness and 98.4% gene annotation completeness. This genomic resource provides a foundation for studying the genetic mechanisms underlying its endangered status and future horticultural.</p>

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Near telomere-to-telomere (T2T) level genome assembly of the critically endangered plant Magnolia zenii (Magnoliaceae)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月8日

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  • Nai-wei Li,
  • Zheng-nan Zhang,
  • Shuai Yuan,
  • Dong-rui Yao,
  • Bao-cheng Wu

摘要

Magnolia zenii (Magnoliaceae) is an extremely endangered tree species endemic to eastern China, has only 115 individuals remaining in the wild. By integrating PacBio HiFi (49×), Oxford Nanopore (ONT; 101×) long-read sequencing, and Hi-C chromatin interaction data, we achieved a high-quality chromosome-level genome with scaffold N50 of 86 Mb, covering 99.97% of the estimated genome size. The genome annotation predicted 74,782 protein-coding genes and revealed a repetitive sequence content of 72.87%, dominated by long terminal repeat (LTR) retrotransposons (53.59%). The mitochondrial (915,126 bp) and chloroplast (160,079 bp) genomes were fully characterized. BUSCO analysis confirmed 99.4% genome completeness and 98.4% gene annotation completeness. This genomic resource provides a foundation for studying the genetic mechanisms underlying its endangered status and future horticultural.