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<p>Histone post-translational modifications (hPTMs) constitute an epigenetic mechanism that plays a fundamental role in the regulation of gene expression in eukaryotic cells. In trypanosomatid parasites, such as <i>Trypanosoma brucei</i>, <i>T. cruzi</i> and <i>Leishmania spp</i>., gene regulation occurs mainly at the post-transcriptional level. Nevertheless, recent studies have demonstrated that hPTMs are abundant and diverse in <i>T. brucei</i> and <i>T. cruzi</i>, the causative agents of sleeping sickness and Chagas disease, respectively, suggesting a potential regulatory role for histone modifications in these organisms. However, data on hPTMs in <i>Leishmania</i> parasites, the etiological agents of leishmaniasis, remain scarce. Here, applying an optimized mass spectrometry-based proteomic workflow, we present the first large-scale, high-confidence map of hPTMs in <i>Leishmania braziliensis</i>, revealing a broad distribution of distinct histone modifications, like what is observed in <i>T. cruzi</i> and other eukaryotes. This dataset provides a valuable resource for future functional studies on chromatin regulation in <i>Leishmania</i> and other trypanosomatids of medical interest.</p>

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Histone post-translational modifications of Leishmania braziliensis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月3日

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  • Rafael Fogaça de Almeida,
  • Matheus Fernandes,
  • Fabiano Borges Figueiredo,
  • Lyris Martins Franco de Godoy

摘要

Histone post-translational modifications (hPTMs) constitute an epigenetic mechanism that plays a fundamental role in the regulation of gene expression in eukaryotic cells. In trypanosomatid parasites, such as Trypanosoma brucei, T. cruzi and Leishmania spp., gene regulation occurs mainly at the post-transcriptional level. Nevertheless, recent studies have demonstrated that hPTMs are abundant and diverse in T. brucei and T. cruzi, the causative agents of sleeping sickness and Chagas disease, respectively, suggesting a potential regulatory role for histone modifications in these organisms. However, data on hPTMs in Leishmania parasites, the etiological agents of leishmaniasis, remain scarce. Here, applying an optimized mass spectrometry-based proteomic workflow, we present the first large-scale, high-confidence map of hPTMs in Leishmania braziliensis, revealing a broad distribution of distinct histone modifications, like what is observed in T. cruzi and other eukaryotes. This dataset provides a valuable resource for future functional studies on chromatin regulation in Leishmania and other trypanosomatids of medical interest.