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<p><i>Agroathelia rolfsii</i> (<i>A. rolfsii</i>) is a globally distributed soilborne fungal pathogen that infects a wide range of crops, causing basal stem rot and yield losses. In this study, we report a high-quality genome assembly of <i>A. rolfsii</i> strain LC1 (43.3 Mb, N50 = 3.3 Mb, GC content = 46.3%) using integrated PacBio long-read and Illumina short-read sequencing technologies. Genome annotation identified 8,826 protein-coding genes, with 30.93% comprising repetitive sequences. Benchmarking Universal Single-Copy Ortholog (BUSCO) analysis demonstrated 97.7% genome completeness, supported by a high QV value of 44, with functional annotations covering 91.1% of the genes. A total of 1,260 carbohydrate-active enzyme (CAZyme) genes, predominantly glycoside hydrolases (37.7%), and 22 secondary metabolite biosynthetic gene clusters were identified, indicating strong host tissue degradation capability and diverse virulence factors. Phylogenetic analysis supported genetic diversity within the <i>Agroathelia</i> genus. This study provides key genomic resources for understanding the pathogenic mechanisms, host interactions, and resistance gene discovery of <i>A. rolfsii</i>, laying the foundation for the development of virulence-targeted control strategies.</p>

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The high-quality genome sequence of Agroathelia rolfsii (Syn. Sclerotium rolfsii), the causal agent of southern blight in Coptis chinensis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月19日

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  • Jingmao You,
  • Lei You,
  • Tao Tang,
  • Fanfan Wang,
  • Houyun Huang,
  • Yuanyuan Duan,
  • Lanlan Zheng,
  • Xiaoyue Wang,
  • Wuxian Zhou,
  • Yonghong Zhang

摘要

Agroathelia rolfsii (A. rolfsii) is a globally distributed soilborne fungal pathogen that infects a wide range of crops, causing basal stem rot and yield losses. In this study, we report a high-quality genome assembly of A. rolfsii strain LC1 (43.3 Mb, N50 = 3.3 Mb, GC content = 46.3%) using integrated PacBio long-read and Illumina short-read sequencing technologies. Genome annotation identified 8,826 protein-coding genes, with 30.93% comprising repetitive sequences. Benchmarking Universal Single-Copy Ortholog (BUSCO) analysis demonstrated 97.7% genome completeness, supported by a high QV value of 44, with functional annotations covering 91.1% of the genes. A total of 1,260 carbohydrate-active enzyme (CAZyme) genes, predominantly glycoside hydrolases (37.7%), and 22 secondary metabolite biosynthetic gene clusters were identified, indicating strong host tissue degradation capability and diverse virulence factors. Phylogenetic analysis supported genetic diversity within the Agroathelia genus. This study provides key genomic resources for understanding the pathogenic mechanisms, host interactions, and resistance gene discovery of A. rolfsii, laying the foundation for the development of virulence-targeted control strategies.