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<p>The genus <i>Ormosia</i> is in the Fabaceae family. China is one of its centers of origin, with almost all species being endemic, suggesting the need for genomic studies to understand its evolution, conservation, and use. Therefore, we generated a chromosome-scale genome assembly of <i>O</i>. <i>boluoensis</i> using Oxford Nanopore long reads combined with Illumina short reads. The initial genome assembly generated by NextDenovo had a total length of 1,638,935,015 bp and a contig N50 of 16,933,043 bp. The assembly after Hi-C reads scaffolding was 1,565,663,440 bp with the scaffold N50 of 201,058,012 bp, and 1,561,022,207 bp (99.70%) for sequences were anchored to 8 chromosomes. Repeats accounted for 75.06% of the assembly. There were 51,822 predicted genes. The BUSCO evaluation showed 98.2% completeness at genome assessment level. The high-quality assembly and annotation provide valuable insights into the genomic characteristics of <i>O</i>. <i>boluoensis</i>, providing resources for its effective conservation and future utilization.</p>

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A chromosome-scale assembly of Ormosia boluoensis (Fabaceae)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月20日

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  • Jin-Pi Zhu,
  • Zheng-Feng Wang,
  • Fang Cheng,
  • En-Ping Yu,
  • Lin Fu,
  • Yan-Na Zeng,
  • Yun-Mei Huang,
  • Xin-Gui Chen,
  • Sheng-Wen Deng,
  • Yue Zhang,
  • Jie-Ming Deng,
  • Hao-Han Lin,
  • Wei-Guang Zhu,
  • Feng-Xia Xu,
  • Hong-Lin Cao

摘要

The genus Ormosia is in the Fabaceae family. China is one of its centers of origin, with almost all species being endemic, suggesting the need for genomic studies to understand its evolution, conservation, and use. Therefore, we generated a chromosome-scale genome assembly of O. boluoensis using Oxford Nanopore long reads combined with Illumina short reads. The initial genome assembly generated by NextDenovo had a total length of 1,638,935,015 bp and a contig N50 of 16,933,043 bp. The assembly after Hi-C reads scaffolding was 1,565,663,440 bp with the scaffold N50 of 201,058,012 bp, and 1,561,022,207 bp (99.70%) for sequences were anchored to 8 chromosomes. Repeats accounted for 75.06% of the assembly. There were 51,822 predicted genes. The BUSCO evaluation showed 98.2% completeness at genome assessment level. The high-quality assembly and annotation provide valuable insights into the genomic characteristics of O. boluoensis, providing resources for its effective conservation and future utilization.