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<p><i>Camellia pitardii</i> Cohen-Stuart, is an endemic winter-spring flowering wild <i>Camellia</i> species in southwestern China, with important ecological and horticultural value. Here, we report the near telomere-to-telomere (T2T) genome of <i>C. pitardii</i> by combining PacBio HiFi, ONT ultra-long, Hi-C and Illumina sequencing platforms. The genome size was 2.63 Gb, with repetitive content of 78.91% and contig N50 of 146.18 Mb. 95.5% of genomic sequences were anchored onto 15 chromosomes, including eight gap-free chromosomes, with 25 telomeres and 15 centromeres. A total of 52,848 protein-coding genes were predicted, of which 98.27% were functionally annotated. The genomic quality was further confirmed by the quality value (QV) of 44.87 and the Benchmarking Universal Single-Copy Orthologs (BUSCO) value of 96.34%. Comparative genomic analysis showed that <i>C. pitardii</i> had a close genetic relationship with <i>C. oleifera</i> and <i>C. chekiangoleosa</i>. The availability of <i>C. pitardii</i> near T2T genome will provide a foundation for identifying key genes related to stress resistance, flowering time regulation, and flower color formation, and promotes the breeding, utilization and conservation of the germplasm resources.</p>

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Near telomere-to-telomere genome assembly of Camellia pitardii

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月14日

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  • Zhiyuan Lv,
  • Jiao Wang,
  • Ling Zhou,
  • Shihui Zou,
  • Lijiao Ai

摘要

Camellia pitardii Cohen-Stuart, is an endemic winter-spring flowering wild Camellia species in southwestern China, with important ecological and horticultural value. Here, we report the near telomere-to-telomere (T2T) genome of C. pitardii by combining PacBio HiFi, ONT ultra-long, Hi-C and Illumina sequencing platforms. The genome size was 2.63 Gb, with repetitive content of 78.91% and contig N50 of 146.18 Mb. 95.5% of genomic sequences were anchored onto 15 chromosomes, including eight gap-free chromosomes, with 25 telomeres and 15 centromeres. A total of 52,848 protein-coding genes were predicted, of which 98.27% were functionally annotated. The genomic quality was further confirmed by the quality value (QV) of 44.87 and the Benchmarking Universal Single-Copy Orthologs (BUSCO) value of 96.34%. Comparative genomic analysis showed that C. pitardii had a close genetic relationship with C. oleifera and C. chekiangoleosa. The availability of C. pitardii near T2T genome will provide a foundation for identifying key genes related to stress resistance, flowering time regulation, and flower color formation, and promotes the breeding, utilization and conservation of the germplasm resources.