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<p>Zoysiagrass stands out as a crucial native turfgrass due to its exceptional abiotic stress tolerance, extensive adaptability, and high ornamental value. In this study, we generated a high-quality chromosome-level genome assembly of Compadre (COM) zoysiagrass, leveraging PacBio SMRT sequencing and Hi-C scaffolding technologies. The resulting genome assembly (312.42 Mb) is anchored on 20 chromosomes, with a Scaffold N50 of 18.72 Mb. In total, 49,074 genes and 306,768 repeat sequences were annotated in the assembled genome. The first chromosome-scale genome of <i>Zoysia japonica</i> ‘Compadre’ provides a critical genetic resource for cold-tolerant turfgrass breeding through identifying stress-responsive candidate genes. Additionally, we have successfully established a cell nucleus extraction and library construction protocol tailored for zoysiagrass ATAC-seq technology, and a total of 80 low temperature tolerance candidate genes were preliminarily identified via ATAC-seq and RNA-seq profiling, thereby initiating the exploration of turfgrass epigenomics.</p>

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Chromosome-scale genome assembly of Zoysia japonica uncovers cold tolerance candidate genes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月3日

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  • Liangying Shen,
  • Zewen Qi,
  • Xiuru Dai,
  • Ye Ai,
  • Jiabao Chen,
  • Yuehui Chao,
  • Hang He,
  • Liebao Han,
  • Lixin Xu

摘要

Zoysiagrass stands out as a crucial native turfgrass due to its exceptional abiotic stress tolerance, extensive adaptability, and high ornamental value. In this study, we generated a high-quality chromosome-level genome assembly of Compadre (COM) zoysiagrass, leveraging PacBio SMRT sequencing and Hi-C scaffolding technologies. The resulting genome assembly (312.42 Mb) is anchored on 20 chromosomes, with a Scaffold N50 of 18.72 Mb. In total, 49,074 genes and 306,768 repeat sequences were annotated in the assembled genome. The first chromosome-scale genome of Zoysia japonica ‘Compadre’ provides a critical genetic resource for cold-tolerant turfgrass breeding through identifying stress-responsive candidate genes. Additionally, we have successfully established a cell nucleus extraction and library construction protocol tailored for zoysiagrass ATAC-seq technology, and a total of 80 low temperature tolerance candidate genes were preliminarily identified via ATAC-seq and RNA-seq profiling, thereby initiating the exploration of turfgrass epigenomics.